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Enter author, analyte, or keyword to search publications.
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| Adler, M., Schulz, S., Spengler, M. (2010) Cytokine Quantification in Drug Development: A comparison of sensitive immunoassay platforms. Chimera Biotech. (Report). Retrieved on 01/26/2010.
ELISA, MSD, ECL, electrochemiluminescence, PCR, comparison, methods
Summary: This report provides a comparison between ELISA, ECL and Immuno-PCR (Imperacer) techniques for quantification of analytes. The main advantages of MSD® platform that have been highlighted include high sensitivity and multiplexing capability
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| Coad, M., Clifford, D., Rhodes, S.G., Hewinson, R.G., Vordermeier, H.M., Whelan, A.O. (2010) Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses. Vet Res. Vol. 41(2):14.
IL10, Interleukin, whole blood, Veterinary Laboratories Agency, IFN-g, IFNg, interferon, IL-1ß, IL1ß, IL1beta, IL4, IL12, MIP1beta, MIP1ß, MIP1b, MIP-1ß, MIP-1b, tumor necrosis factor, TNF-a, TNFa, TNFalpha, TNF-a, TNFa
Analytes tested using MSD assays: IFN-gamma, IL-1beta, IL-4, IL-10, IL-12, MIP-1beta and TNF-alpha
Matrix tested: antigen stimulated bovine whole blood cultures on custom bovine 7-plex panel
Summary: This paper is based on a study conducted by VLA to study the diagnostic and immunological consequences of repeated skin tests in cattle infected with Mycobacterium bovis.
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| Crotta, S., Brazzoli, M., Piccioli, D., Valiante, N.M., Wack, A. (2010) Hepatitis C virions subvert natural killer cell activation to generate a cytokine environment permissive for infection. J Hepatol. Vol. 52(2):183-90.
IL8, Interleukin, Interferon, IFN, IFNgamma, IFNg, IFN-g, IFN-g, IFNg, natural killer cells
Analytes tested using MSD assays: IFN-gamma, IL-8
Matrix tested: Human NK cell culture supernatants
Summary: This paper investigates whether Hepatitis C viral infection affects activation of natural killer cells and the impact of altered NK cell activation on the overall infectivity of the virus.
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| Fu, Q., Zhu, J., Van Eyk, J.E. (2010) Comparison of Multiplex Immunoassay Platforms. Clinical Chemistry. Vol. 56(2):314–318.
IL1b, IL1beta, IL-1b, IL1-b, Interleukin, IL2, IL6, IL10, IL12p70, IL12-p70, IL-12(p70)
Analytes tested using MSD assays: IL-1beta, IL-2, IL-6, IL-10, IL-12p70
Matrix tested: Human serum
Summary: In this paper, five different multiplex immunoassays were compared for reproducibility, robustness, accuracy and throughput (MESO SCALE DISCOVERY® technology, FAST Quant, A2 from Beckman Coulter, Bio-Plex from Bio-Rad, FlowCytomix from Bender MedSystems).
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| Gadjeva, M., Paradis-Bleau, C., Priebe, G.P., Fichorova, R., Pier, G.B. (2010) Caveolin-1 modifies the immunity to Pseudomonas aeruginosa. J Immunol. Vol. 184(1):296-302.
IL1b, IL1beta, IL-1b, IL1-b, Interleukin, IL2, IL6, IL10, IL12p70, IL12-p70, IL-12(p70), Macrophage migration inhibitory factor
Analytes tested using MSD assays: IL-1beta, IL-6, IL-12p70, IL-10, IFN-gamma, KC / CXCL1 / GRO
Matrix tested: Mouse cornea lysates
Summary: In this paper, the effect of inhibition of MIF on Pseudomonas aeruginosa induced corneal infections was tested in mice.
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| Gordon, M.S., Sweeney, C.S., Mendelson, D.S., Eckhardt, S.G., Anderson, A., Beaupre, D.M., Branstetter, D., Burgess, T.L., Coxon, A., Deng, H., Kaplan-Lefko, P., Leitch, I.M., Oliner, K.S., Yan, L., Zhu, M., Gore, L. (2010) Safety, pharmacokinetics, and pharmacodynamics of AMG 102, a fully human hepatocyte growth factor-neutralizing monoclonal antibody, in a first-in-human study of patients with advanced solid tumors. Clin Cancer Res. Vol. 16(2):699-710.
Hepatocyte growth factor, scatter factor, cMet
Analytes tested using MSD assays: Soluble c-Met
Matrix tested: Human plasma
Summary: Safety, anti–AMG 102 antibody formation, pharmacokinetics, tumor response, and exploratory biomarkers were assessed in patients with refractory advanced solid tumors.
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| Gouin, J.P., Carter, C.S., Pournajafi-Nazarloo, H., Glaser, R., Malarkey, W.B., Loving, T.J., Stowell, J., Kiecolt-Glaser, J.K. (2010) Marital behavior, oxytocin, vasopressin, and wound healing. Psychoneuroendocrinology. [Epub ahead of print].
Interleukin, IL6, Tumor necrosis factor, TNFalpha, TNFa, TNF-a, TNF-a, TNFa, Oxytocin, Vasopressin
Analytes tested using MSD assays: IL-6, TNF-alpha
Matrix tested: Human plasma
Summary: In this study, the relationships between marital behavior and endogenous oxytocin and vasopressin were examined to determine the extent to which these peptides modulate proinflammatory cytokine production and wound healing, and to evaluate the characteristics of individuals with the highest neuropeptide levels.
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| Janot-Sardet, C., Assouline, B., Cheynier, R., Morre, M., Beq, S. (2010) A validated assay to measure soluble IL-7 receptor shows minimal impact of IL-7 treatment. J Immunol Methods. Vol. 353(1-2):115-23.
Interleukin, IL-7, IL7, HIV, simian
Analytes tested using MSD assays: Customer developed assay for soluble CD127
Matrix tested: Human plasma from normal and HIV-infected patients, Rhesus plasma, Cynomolgus plasma
Summary: In this paper, the development of a validated assay for the measurement of soluble IL-7 receptor alpha chain (CD127) in human and simian plasma using MSD technology has been described.
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| Jorde, R., Sneve, M., Torjesen, P.A., Figenschau, Y., Gøransson, L.G., Omdal, R. (2010) No effect of supplementation with cholecalciferol on cytokines and markers of inflammation in overweight and obese subjects. Cytokine. Vol. 50(2):175-80.
Interleukin, intercellular adhesion molecule-1, monocyte chemotactic protein-1, IL2, IL4, IL5, IL10, IL12, IL13, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, IL17, ICAM1, MCP1
Analytes tested using MSD assays: TH1/TH2 7-plex (IL-2, IL-4, IL-5, IL-10, IL-12, IL-13, IFN-gamma), IL-17, ICAM-1, MCP-1
Matrix tested: Human serum
Summary: In this paper, the effect of serum levels of vitamin D on cytokines and inflammation markers was tested in 437 overweight subjects with and without supplementation of the vitamin.
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| Khamri, W., Walker, M.M., Clark, P., Atherton, J.C., Thursz, M.R., Bamford, K.B., Lechler, R.I., Lombardi, G. (2010) Helicobacter pylori Stimulates Dendritic Cells to Induce IL-17 Expression From CD4+ T lymphocytes. Infect Immun. Vol. 78(2):845-53.
IL-1b, IL-1ß, IL1ß, IL1beta, Interleukin
Analytes tested using MSD assays: IL-1beta
Matrix tested: Human Peripheral blood mononuclear cell (PBMC) supernatants
Summary: In this paper, the role of dendritic cells in the induction of immune responses to Helicobacter pylori infection has been studied.
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| Li, P., Lu, M., Nguyen, M.T., Bae, E., Chapman, J., Feng, D., Hawkins, M., Pessin, J.E., Sears, D.D., Nguyen, A.K., Amidi, A., Watkins, S.M., Nguyen, U., Olefsky, J.M. (2010) Functional heterogeneity of CD11C positive adipose tissue macrophages in diet-induced obese mice. J Biol Chem. Vol. 285(20):15333-45.
Interleukin, IL6, IL10, IL12p70, GRO / CXCL1, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, tumor necrosis factor, TNFa, TNF-a, TNFalpha, TNFa, TNF-a
Analytes tested using MSD assays: IL-12p70, IFN-gamma, IL-6, IL-10, KC and TNF-alpha
Matrix tested: Mouse Epididymal White Adipose Tissue lysates
Summary: In this paper, the number of CD11c positive Adipose Tissue Macrophages and their phenotype was studied in insulin sensitive mice after a diet change.
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| Moussaud, S., Draheim, H.J. (2010) A new method to isolate microglia from adult mice and culture them for an extended period of time. J Neurosci Methods. Vol. 187(2):243-253.
Interleukin, IL2, IL4, IL5, IL10, IL12, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, GRO / CXCL1
Analytes tested using MSD assays: Mouse Th1/Th2 9-Plex (IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, KC / GRO / CINC, IL-10, IL-12, TNF-alpha)
Matrix tested: Mouse microglial cell culture supernatants
Summary: In this study, a new method for the isolation and culture of an almost pure population of microglia from adult mouse brains has been described.
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| Rask, C , Brimnes, J., Lund, K. (2010) Shorter dosing intervals of sublingual immunotherapy lead to more efficacious treatment in a mouse model of allergic inflammation. Scandinavian Journal of Immunology.
Interleukin, IL2, IL4, IL5, IL10, IL12, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, GRO / CXCL1
Analytes tested using MSD assays: Mouse Th1/Th2 9-Plex (IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, KC / GRO / CINC, IL-10, IL-12, TNF-alpha)
Matrix tested: Mouse cell culture supernatants
Summary: In this study, the immunological effect of increased SLIT dosing frequency was tested in a mouse model of allergic inflammation.
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| Rizzetto, L., Kuka, M., De Filippo, C., Cambi, A., Netea, M.G., Beltrame, L., Napolitani, G., Torcia, M.G., D'Oro, U., Cavalieri, D. (2010) Differential IL-17 production and mannan recognition contribute to fungal pathogenicity and commensalism. J Immunol. Vol. 184(8):4258-68.
Interleukin, IL6, IL8, IL10, IL12p70, tumor necrosis factor, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL1beta, IL-1b, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: IL-1beta, IL-6, IL-8, IL-10, IL-12p70, TNF-alpha
Matrix tested: Peripheral blood mononuclear cell (PBMC) supernatants
Summary: In this study, differential Th17 responses in dendritic cells exposed to pathogenic and non-pathogenic fungi have been investigated and the role of cell wall mannan in fungal immune response has been studied in detail.
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| Saric, J., Li, J.V., Swann, J., Utzinger, J., Calvert, G., Nicholson, J.K., Dirnhofer, S., Dallman, M., Bictash, M., Holmes, E. (2010) Integrated Cytokine and Metabolic Analysis of Pathological Responses to Parasite Exposure in Rodents. J Proteome Res. Vol. 9(5):2255-64.
Interleukin, IL2, IL4, IL5, IL10, IL12, IL13, GRO / CXCL1, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, tumor necrosis factor, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: Mouse TH1/TH2 7-Plex (IL-1beta, IL-2, IL-4, IL-5, IL-10, IL-12, IFN-gamma, KC / GRO, TNF-alpha), Rat Demonstration Panel (IFN-gamma, IL-1beta, IL-4, IL-5, IL-13, KC / GRO, TNF-alpha)
Matrix tested: Mouse and Rat Plasma
Summary: In this paper, cytokine profiles of four parasite-rodent models were tested in relation to their metabolic phenotypes.
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| Suen, K.F., Turner, M.S., Gao, F., Liu, B., Althage, A., Slavin, A., Ou, W., Zuo, E., Eckart, M., Ogawa, T., Yamada, M., Tuntland, T., Harris, J.L., Trauger, J.W. (2010) Transient expression of an IL-23R extracellular domain Fc fusion protein in CHO vs. HEK cells results in improved plasma exposure. Protein Expr Purif. Vol. 71(1):96-102.
Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g
Analytes tested using MSD assays: IFN-gamma
Matrix tested: Human peripheral blood mononuclear cell (PBMC) supernatants
Summary: The differences in plasma exposure and in vivo activity of IL-23R Fc fusion protein were correlated with its expression in different cell lines (CHO vs HEK).
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| Sugimoto, H., Okada, K., Shoda, J., Warabi, E., Ishige, K., Ueda, T., Taguchi, K., Yanagawa, T., Nakahara, A., Hyodo, I., Ishii, T., Yamamoto, M. (2010) Deletion of nuclear factor-E2-related factor-2 leads to rapid onset and progression of nutritional steatohepatitis in mice. Am J Physiol Gastrointest Liver Physiol. Vol. 298(2):G283-94.
Monocyte chemoattractant protein 1
Analytes tested using MSD assays: Adiponectin, MCP-1
Matrix tested: Mouse serum
Summary: The role of Nrf2, which serves as a cellular stress sensor, in protection against steatohepatitis has been investigated in this paper.
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| Szeto, G.L., Brice, A.K., Yang, H.C., Barber, S.A., Siliciano, R.F., Clements, J.E. (2010) Minocycline attenuates HIV infection and reactivation by suppressing cellular activation in human CD4+ T cells. J Infect Dis. Vol. 201(8):1132-40.
Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, Interleukin, IL2, TNF-a, TNFa, TNFa, TNF-a, tumor necrosis factor, TNFalpha
Analytes tested using MSD assays: IFN-gamma, IL-2, TNF-alpha
Matrix tested: Human cell culture supernatants
Summary: This study investigates the mechanism of action of anti-HIV drug, minocycline, which reduces viral replication by altering the cellular environment rather than targeting the virus directly.
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| Young, J., Yu, X., Wolslegel, K., Nguyen, A., Kung, C., Chiang, E., Kolumam, G., Wei, N., Wong, W.L., DeForge, L., Townsend, M.J., Grogan, J.L. (2010) Lymphotoxin-aß heterotrimers are cleaved by metalloproteinases and contribute to synovitis in rheumatoid arthritis. Cytokine 2010.
TNF-a, TNFa, TNFalpha, TNF-a, TNFa, tumor necrosis factor, Interleukin, IL-1b, IL1b, IL1ß. IL-1ß, IL1beta, IL6, IL8, IL12p70, Interferon, IFNgamma, IFN-g, IFNg, IFN-g, IFNg, rheumatoid arthritis, synovium
Analytes tested using MSD assays: TNF-alpha, Lymphotoxin alpha/beta, IL-1beta, IFN-gamma, IL-12p70, IL-6, IL-8, IL-10
Matrix tested: Human serum and synovial fluid
Summary: This study determines whether Lymphotoxin alpha/beta heterotrimers (members of TNF-superfamily) are cleaved from cell membranes and are active in rheumatoid arthritis.
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| Zhao, M., Wigren, M., Dunér, P., Kolbus, D., Olofsson, K.E., Björkback, H., Nilsson, J., Fredrikson, G.N. (2010) FcgammaRIIB inhibits the development of atherosclerosis in low-density lipoprotein receptor-deficient mice. J Immunol. Vol. 184(5):2253-60.
FcgRIIB, FcgRIIB, TH1, TH2, Interleukin, IL2, IL4, IL5, IL10, IL12, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: Mouse Th1/Th2 9-plex Ultra-Sensitive Kit (IFN-gamma, IL-1beta, TNF-alpha, IL-2, IL-12, IL-4, IL-5, IL-10, KC / GRO / CINC)
Matrix tested: Mouse plasma, mouse splenocyte cell culture supernatants
Summary: This study examines the role of the inhibitory IgG receptor Fc(gamma)RIIB in atherosclerosis.
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| Bafadhel, M., Saha, S., Siva, R., McCormick, M., Monteiro, W., Rugman, P., Dodson, P., Pavord, I.D., Newbold, P., Brightling, C.E. (2009) Sputum IL-5 Concentration Is Associated with a Sputum Eosinophilia and Attenuated by Corticosteroid Therapy in COPD. Respiration. Vol. 78:256-262.
Interleukin, IL4, IL5, IL6, IL10, IL13, IL17, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, Chronic obstructive pulmonary disease
Analytes tested using MSD assays: IL-1beta, IL-4, IL-5, IL-6, IL-10, IL-13, IL-17, IFN-gamma, TNF-a, CD120a, CD120b, CD126, GM-CSF, CCL2, CCL4, CCL3, CCL5, CCL11, CCL13, CCL17, CCL22, CCL26, CXCL8, CXCL10, CXCL11, VEGF
Matrix tested: Human sputum
Summary: In this paper, sputum cytokine and chemokine profile was tested in COPD patients with or without eosinophilia for biomarker validation.
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| Bollyky, P.L., Falk, B.A., Long, S.A., Preisinger, A., Braun, K.R., Wu, R.P., Evanko, S.P., Buckner, J.H., Wight, T.N., Nepom, G.T. (2009) CD44 costimulation promotes FoxP3+ regulatory T cell persistence and function via production of IL-2, IL-10, and TGF-beta. J Immunol. Vol. 183(4):2232-41.
Interleukin, IL4, IL5, IL10, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: IL-1beta, IL-4, IL-5, IL-10, IFN-gamma, TNF-a
Matrix tested: Human PBMC and mouse leukocyte cell culture supernatants
Summary: In this paper, the mechanism of the interaction between extracellular matrix receptor (CD44) and its ligand Hyaluronan (HA) has been studied with regard to regulatory T cell function.
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| Brignone, C., Escudier, B., Grygar, C., Marcu, M., Triebel, F. (2009) A phase I pharmacokinetic and biological correlative study of IMP321, a novel MHC class II agonist, in patients with advanced renal cell carcinoma. Clin Cancer Res. Vol. 15(19):6225-31.
Interleukin, IL6, IL8, IP10, MCP1, MCP4, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, MIP-1b, MIP1b, MIP-1ß, MIP1ß, MIP1beta, monocyte chemotactic protein, Macrophage-Derived Chemokine, Thymus and Activation Regulated Chemokine, Interferon Inducible Protein 10, tumor necrosis factor
Analytes tested using MSD assays: Human ProInflammatory 4-plex (IFN-gamma, IL-1beta, IL-6, TNF-alpha), and Human Chemokine 9-plex (Eotaxin, Eotaxin-3, IL-8, IP-10, MCP-1, MCP-4, MDC, MIP-1beta, and TARC)
Matrix tested: Human plasma
Summary: In this paper, the pharmacokinetics of an anti-cancer drug, IMP321, in a Phase I clinical trial has been studied.
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| Cardin, R.D., Bravo, F.J., Sewell, A.P., Cummins, J., Flamand, L., Juteau, J.M., Bernstein, D.I., Vaillant, A. (2009) Amphipathic DNA polymers exhibit antiviral activity against systemic murine Cytomegalovirus infection. Virol J. Vol. 6:214.
Interleukin, IL2, IL4, IL5, IL6, IL8, IL10, IL12p70, IL13, IP10, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, Interferon Inducible Protein 10, tumor necrosis factor
Analytes tested using MSD assays: IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, TNF-alpha, IP-10
Matrix tested: Human peripheral blood mononuclear cell (PBMC) supernatants
Summary: Here, the in vivo effect of phosphorothioated oligonucleotides against Cytomegaloviral infections was studied.
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| Chiang, P.C., Hu, Y., Thurston, A., Sommers, C.D., Guzova, J.A., Kahn, L.E., Lai, Y., Blom, J.D. (2009) Pharmacokinetic and pharmacodynamic evaluation of the suitability of using fluticasone and an acute rat lung inflammation model to differentiate lung versus systemic efficacy. J Pharm Sci. Vol. 98(11):4354 -4364.
TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, lung inflammation, glucocorticoids, PK/PD
Analytes tested using MSD assays: TNF-alpha
Matrix tested: Rat bronchoalveolar lavage fluid (BALF)
Summary: In this study, the efficacy of inhaled fluticasone has been examined with respect to that arising from local pulmonary exposures as compared to systemic exposure. Different formulations of fluticasone were used to demonstrate lung targeted efficacy in the lipopolysaccharide induced inflammation rat model.
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| Chowdhury, F., Williams, A. (2009) From research to regulated: challenges in transferring methods. Bioanalysis. Vol. 1(2):285-291.
Luminex, MESO SCALE DISCOVERY, clinical
Summary: This report summarizes some of the challenges as well as the solutions that academic units have in order to successfully transfer techniques from a research laboratory to a clinical setting for phase I trials.
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| Chowdhury. F., Williams, A., Johnson, P. (2009) Validation and comparison of two multiplex technologies, Luminex® and Mesoscale Discovery, for human cytokine profiling. J Immunol Methods. Vol. 340(1):55-64.
Interleukin, IL2, IL4, IL5, IL8, IL10, IL12p70, IL13, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, tumor necrosis factor
Analytes tested using MSD assays: Human TH1/TH2 10-Plex (IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, IL-8, IL-10, IL-12p70, IL-13, TNF-alpha)
Matrix tested: Human plasma
Summary: Two multiplex platforms have been directly compared here for measurement of human cytokines in ex-vivo stimulated whole blood.
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| Christensen, E., Pintilie, M., Evans, K.R., Lenarduzzi, M., Menard, C., Catton, C.N., Diamandis, E.P., Bristow, R.G. (2009) Longitudinal cytokine expression during IMRT for prostate cancer and acute treatment toxicity. Clin Cancer Res. Vol. 15(17):5576-83.
Interleukin, IL2, IL6, IL8, IL10, IL12p70, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, granulocyte macrophage colony stimulating factor, tumor necrosis factor
Analytes tested using MSD assays: GM-CSF, IFN-gamma, IL-1beta, IL-2, IL-6, IL-8, IL-10, IL-12p70, TNF-alpha
Matrix tested: Human serum
Summary: This paper studies the expression of specific cytokines correlating with differential toxicity in prostrate cancer patients undergoing radiation therapy.
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| Christian, L.M., Franco, A., Glaser, R. Iams, J.D. (2009) Depressive symptoms are associated with elevated serum proinflammatory cytokines among pregnant women. Brain, Behavior, and Immunity. Vol. 23 (6):750-754.
Interleukin, IL6, tumor necrosis factor, TNFa, TNF-a, TNFalpha, TNFa, TNF-a
Analytes tested using MSD assays: IL-6, TNF-alpha
Matrix tested: Human serum
Summary: This study examines the associations between stress, depressive symptoms, and serum inflammatory markers in a sample of 60 pregnant women to test whether inflammation is a likely mechanism by which distress can cause negative perinatal outcomes.
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| Chung, D.J., Rossi, M., Romano, E., Ghith, J., Yuan, J., Munn, D.H., Young, J.W. (2009) Indoleamine 2,3-dioxygenase–expressing mature human monocyte-derived dendritic cells expand potent autologous regulatory T cells. Blood. Vol. 114(3):555-563.
Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g
Analytes tested using MSD assays: IFN-gamma
Matrix tested: Human leukocyte cell culture supernatants
Summary: In this paper, an Indoleamine 2,3-dioxygenase (IDO) dependent mechanism of regulatory T cell (Tregs) expansion by dendritic cells has been identified and discussed in detail.
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| Clarke, S., Laxton, C., Horscroft, N., Richard, V., Thomas, A., Parkinson, T. (2009) Comparison of Rat and Human Responses to Toll-Like Receptor 7 Activation. J Interferon Cytokine Res. Vol. 29(2):113-26.
Interleukin, IL6, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, Interferon, Interferon alpha 2a, IFNa2a, IFN-a2a, IFN-a2a, IFNa2a, interferon-alpha-2a
Analytes tested using MSD assays: IL-1beta, IL6, TNF-alpha, IFN-alpha2a
Matrix tested: Human and rat peripheral blood mononuclear cell (PBMC) culture supernatants
Summary: In this paper, the responses to Toll like receptor 7 (TLR7) agonist, SM360320, was investigated in rats and human PBMCs, and compared to the responses observed in whole animals.
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| Cockcroft, N.Y., Oke, O., Cunningham, F., Bishop, E., Fearon, I.M., Zantl, R., Gaca, M.D. (2009) An in vitro perfusion system to examine the responses of endothelial cells to simulated flow and inflammatory stimulation. Altern Lab Anim. Vol. 37(6):657-69.
IL6, IL8, Interleukin, monocyte chemotactic protein-1, MCP1
Analytes tested using MSD assays: IL-6, IL-8, MCP-1
Matrix tested: Human umbilical vein endothelial cell (HUVEC) culture medium
Summary: In this study, a simulated blood flow perfusion system was developed in vitro to test the effect of different flow patterns on the formation of atherosclerotic lesions.
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| Di Sabatino, A., Rovedatti, L., Kaur, R., Spencer, J.P., Brown, J.T., Morisset, V.D., Biancheri, P., Leakey, N.A., Wilde, J.I., Scott, L., Corazza, G.R., Lee, K., Sengupta, N., Knowles, C.H., Gunthorpe, M.J., McLean, P.G., MacDonald, T.T., Kruidenier, L. (2009) Targeting gut T cell Ca2+ release-activated Ca2+ channels inhibits T cell cytokine production and T-box transcription factor T-bet in inflammatory bowel disease. J Immunol. Vol. 183(5):3454-62.
Interleukin, IL2, IL8, IL17, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g
Analytes tested using MSD assays: IFN-gamma, IL-2, IL-8, IL-17
Matrix tested: Cell culture supernatants from human endoscopic biopsy and surgical specimens
Summary: This paper proposes the Ca(2+) release-activated Ca(2+) (CRAC) channel as a potential therapeutic target for the treatment of inflammatory bowel disease (IBD) and characterizes the use of a CRAC channel inhibitor, Synta 66, to inhibit T cell cytokine production, T-bet expression and proliferation of T cells in gut-derived lamina propria mononuclear cells (LPMCs) and organ cultures of IBD biopsy specimens.
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| Enewold, L., Mechanic, L.E., Bowman, E.D., Zheng, Y.L., Yu, Z., Trivers, G., Alberg, A.J., Harris, C.C. (2009) Serum concentrations of cytokines and lung cancer survival in African Americans and Caucasians. Cancer Epidemiol Biomarkers Prev. Vol. 18(1):215-22.
Interleukin, IL4, IL5, IL6, IL8, IL10, IL12p70, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, granulocyte macrophage colony stimulating factor
Analytes tested using MSD assays: GM-CSF, IFN-gamma, IL-1beta, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, TNF-alpha
Matrix tested: Human serum
Summary: This paper shows the results of a case-control study in which the association between different proinflammatory and anti-inflammatory cytokines with lung cancer survival has been tested.
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| Frick, J.S., MacManus, C.F., Scully, M., Glover, L.E., Eltzschig, H.K., Colgan, S.P. (2009) Contribution of adenosine A2B receptors to inflammatory parameters of experimental colitis. J Immunol. Vol. 182(8):4957-64.
Interleukin, IL2, IL4, IL5, IL10, IL12, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, KC/GRO/CINC, IL-10, IL-12, TNF-a
Matrix tested: Mouse colon lysates
Summary: In this study, the role of Adenosine receptor (A2B) expressed on murine intestinal epithelial cells on experimentally induced colitis was tested.
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| Friese, R.S., Rao, F., Khandrika, S., Thomas, B., Ziegler, M.G., Schmid-Schönbein, G.W., O'Connor, D.T. (2009) Matrix metalloproteinases: discrete elevations in essential hypertension and hypertensive end-stage renal disease. Clin Exp Hypertens. Vol. 31(7):521-33.
matrix metalloproteinase
Analytes tested using MSD assays: MMP-1, MMP-2, MMP-3, MMP-10
Matrix tested: Human plasma
Summary: In this paper, the role of MMPs as inflammatory mediators in complicated as well as uncomplicated hypertension was studied.
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| Getz, E.B., Fisher, D.M., Fuller, R. (2009) Human pharmacokinetics/pharmacodynamics of an interleukin-4 and interleukin-13 dual antagonist in asthma. J Clin Pharmacol. Vol. 49(9):1025-36.
pitrakinra, IL4, Interleukin
Analytes tested using MSD assays: Customer developed assay for a drug
Matrix tested: Human plasma
Summary: The pharmacokinetics of a human IL-4 mutein that binds to IL-4 receptor was studied in this paper and compared to the pharmacological response in asthma.
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| Gourh, P., Arnett, F.C., Assassi, S., Tan, F.K., Huang, M., Diekman, L., Mayes, M.D., Reveille, J.D., Agarwal, S.K. (2009) Plasma cytokine profiles in systemic sclerosis: associations with autoantibody subsets and clinical manifestations. Arthritis Res Ther. Vol. 11(5):R147.
Interleukin, IL2, IL4, IL5, IL6, IL8, IL10, IL12p70, IL13, IL17, IL23, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: IFN-gamma, TNF-alpha, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17, IL-23
Matrix tested: Human plasma
Summary: This study determines whether plasma cytokine profiles differ in systemic sclerosis patients grouped according to the three different SSc associated autoantibody subsets, anticentromere antibodies (ACAs), anti-topoisomerase antibodies (ATAs), and anti-RNA polymerase III antibodies (ARAs). The role of MMPs as inflammatory mediators in complicated as well as uncomplicated hypertension was studied.
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| Grip, O., Janciauskiene, S. (2009) Atorvastatin reduces plasma levels of chemokine (CXCL10) in patients with Crohn's disease. PLoS ONE. Vol. 4(5):5263.
IL8, IP10, Interleukin, IL8, IP10, MCP1, MCP4, MIP-1b, MIP1b, MIP-1ß, MIP1ß, MIP1beta, monocyte chemotactic protein, Macrophage-Derived Chemokine, Thymus and Activation Regulated Chemokine, Interferon Inducible Protein 10
Analytes tested using MSD assays: MCP-1, MIP-1beta, Eotaxin, MCP-4, TARC, MDC, Eotaxin-3, IL-8, IP-10, P-selectin,, E-selectin, sICAM-3, Thrombomodulin
Matrix tested: Human plasma
Summary: Plasma levels of cytokines and chemokines in Crohn’s disease patients administered with statins were measured to determine whether chemokine expression reduces after statin treatment.
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| Hu, Y.L., Metz, D.P., Chung, J., Siu, G., Zhang, M. (2009) B7RP-1 blockade ameliorates autoimmunity through regulation of follicular helper T cells. J Immunol. Vol. 182(3):1421-8.
Interferon, IFN-g, IFNg, IFNgamma, IFN-g, IFNg
Analytes tested using MSD assays: IFN-gamma
Matrix tested: Mouse lymphocyte cell culture medium
Summary: This paper discusses the critical role played by ICOS and its ligand, B7RP-1, in enhancing the development of autoimmune diseases in murine models of rheumatoid arthritis and systemic lupus erythematosus.
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| Keely, S., Ryan, S.M., Haddleton, D.M., Limer, A., Mantovani, G., Murphy, E.P., Colgan, S.P., Brayden, D.J. (2009) Dexamethasone-pDMAEMA polymeric conjugates reduce inflammatory biomarkers in human intestinal epithelial monolayers. J Control Release. Vol. 135(1):35-43.
Interleukin, IL2, IL4, IL5, IL8, IL10, IL12p70, IL13, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: Human TH1/TH2 10-Plex (IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, IL-8, IL-10, IL-12p70, IL-13, TNF-alpha)
Matrix tested: Human cell culture supernatants (Caco-2)
Summary: In this paper, a mucoadhesive polymer, pDMAEMA, was synthesized and conjugated to dexamethasone (DEX) and the adhesive as well as anti-inflammatory properties of this conjugate (DEX-pDMAEMA) was studied in human intestinal monolayers.
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| Keun, H.C., Sidhu, J., Pchejetski, D., Lewis, J.S., Marconell, H., Patterson, M., Bloom, S.R., Amber, V., Coombes, R.C., Stebbing, J. (2009) Serum molecular signatures of weight change during early breast cancer chemotherapy. Clin Cancer Res. Vol. 15(21):6716-23.
IL6, Interleukin, TNFa, TNF-a, TNFalpha, TNFa, tumor necrosis factor, TNF-a
Analytes tested using MSD assays: IL-6, TNF-alpha
Matrix tested: Human serum
Summary: In this paper, metabolic and cytokine profiles of breast cancer patients undergoing chemotherapy was studied to gain insights into the physiologic processes associated with weight gain in these patients.
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| Kleinschek, M.A., Boniface, K., Sadekova, S., Grein, J., Murphy, E.E., Turner, S.P., Raskin, L., Desai, B., Faubion, W.A., de Waal Malefyt, R., Pierce, R.H., McClanahan, T., Kastelein, R.A. (2009) Circulating and gut-resident human Th17 cells express CD161 and promote intestinal inflammation. J Exp Med. Vol. 206(3):525-34.
Interferon, Interleukin, IL17, IL17F, IFNgamma, IFN-g, IFNg, IFNg, IFN-g
Analytes tested using MSD assays: IFN-gamma, IL-17, IL-17F
Matrix tested: Human cell culture supernatants
Summary: This study identified that there exists an abundance of TH17 cells expressing CD161+ receptor in the intestines of Crohn’s disease patients and this Th17 cell pool can be activated by IL-23 to cause destructive tissue inflammation.
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| Koppe, S.W., Elias, M., Moseley, R.H., Green, R.M. (2009) Trans fat feeding results in higher serum alanine aminotransferase and increased insulin resistance compared with a standard murine high-fat diet. Am J Physiol Gastrointest Liver Physiol. Vol. 297(2):G378-84.
IL-1b, IL-1ß, IL1ß, IL1beta, Interleukin
Analytes tested using MSD assays: Insulin, Leptin, IL-1beta
Matrix tested: Mouse serum and liver / hepatic tissue lysates
Summary: In this paper, the effect of high trans fat diet on the development of nonalcoholic fatty liver disease was examined and compared to a high fat diet devoid of trans fats.
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| Kuai, J., Gregory, B., Hill, A., Pittman, D.D., Feldman, J.L., Brown, T., Carito, B., O'Toole, M., Ramsey, R., Adolfsson, O., Shields, K.M., Dower, K., Hall, J.P., Kurdi, Y., Beech, J.T., Nanchahal, J., Feldmann, M., Foxwell, B.M., Brennan, F.M., Winkler, D.G., Lin, L.L. (2009) TREM-1 expression is increased in the synovium of rheumatoid arthritis patients and induces the expression of pro-inflammatory cytokines. Rheumatology (Oxford). Vol. 48(11):1352-8.
Triggering receptor expressed on myeloid cells-1, TREM1, rheumatoid arthritis, Interleukin, IL-1b, IL-1ß, IL1ß, IL1beta, IL8, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, Granulocyte Macrophage Colony Stimulating Factor
Analytes tested using MSD assays: IL-1beta, IL-8, GM-CSF, TNF-alpha
Matrix tested: Human synovium cell culture supernatants from Rheumatoid arthritis patients
Summary: In this paper, the expression and the role of TREM-1 in the synovium of rheumatoid arthritis patients was investigated and the level of TREM-1 in plasma of these patients was assessed.
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| Kuhle, J., Lindberg, R.L., Regeniter, A., Mehling, M., Steck, A.J., Kappos, L, Czaplinski, A. (2009) Increased levels of inflammatory chemokines in amyotrophic lateral sclerosis. Eur J of Neurology. Vol. 16(6):771-4.
chemokine, IL8, Interleukin, IP10, Macrophage Derived Chemokine, Macrophage Inflammatory Protein, Thymus and Activation Regulated Chemokine, Interferon Inducible Protein, Monocyte Chemotactic Protein
Analytes tested using MSD assays: Eotaxin, Eotaxin-3, IL-8, IP-10, MCP-1, MCP-4, MDC, MIP-1beta, TARC
Matrix tested: Human serum and cerebrospinal fluid (CSF)
Summary: In this paper, the levels of inflammatory markers in amyotrophic lateral sclerosis were studied in comparison to other non-inflammatory neurological diseases.
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| Lembo, A., J, Neri, B., Tolley, J., Barken, D., Carroll, S., Pan, H. (2009) Use of serum biomarkers in a diagnostic test for irritable bowel syndrome. Aliment Pharmacol Ther. Vol. 29(8):834-42.
Interleukin, IL1ß, IL-1ß, IL1b, IL1beta, IL-1b, Brain derived neurotrophic factor, TNF-alpha like weak inducer of apoptosis, Tissue Inhibitor of Metalloproteinase-1, Neutrophil gelatinase associated lipocalin, Growth-related oncogene- a, IBS
Analytes tested using MSD assays: IL-1beta, BDNF, TWEAK, TIMP-1, NGAL, GRO-a
Matrix tested: Human serum
Summary: In this study, a set of 10 biomarkers were selected out of a panel of 140 markers to develop a diagnostic test for Irritable Bowel Syndrome.
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| Lindqvist, D., Janelidze, S., Hagell, P., Erhardt, S., Samuelsson, M., Minthon, L., Hansson, O., Björkqvist, M., Träskman-Bendz, L., Brundin, L. (2009) Interleukin-6 Is Elevated in the Cerebrospinal Fluid of Suicide Attempters and Related to Symptom Severity. Biol Psychiatry. Vol. 66(3):287-92.
CSF, Interleukin, IL1ß, IL-1ß, IL1b, IL1beta, IL6, IL8, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, major depressive disorder, depression
Analytes tested using MSD assays: IL-1beta, IL-6, IL-8, TNF-alpha
Matrix tested: Human cerebrospinal fluid, human plasma
Summary: In this study, cytokines in cerebrospinal fluid of suicide attempters were measured to identify immune system alterations associated with depressive disorders.
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| Liu, Y., Zhu, L., Fatheree, N.Y., Liu, X., Pacheco, S.E., Tatevian, N., Rhoads, J.M. (2009) Changes in Intestinal Toll-like Receptors and Cytokines Precede Histological Injury in a Rat Model of Necrotizing Enterocolitis. Am J Physiol Gastrointest Liver Physiol. Vol. 297(3):442-50.
Interleukin, IL1ß, IL-1ß, IL1b, IL1beta, IL-1b, keratinocyte derived chemokine / growth related oncogene, IL4, IL5, IL13, TNFa, TNF-a, TNFalpha, tumor necrosis factor, TNFa, TNF-a
Analytes tested using MSD assays: IFN-gamma, IL-1beta, TNF-alpha, KC / GRO/ CXCL1, IL-4, IL-5, IL-13
Matrix tested: Rat ileal tissue lysates
Summary: The role of Toll-like receptor signaling and cytokine expression in the pathogenesis of neonatal necrotizing enterocolitis has been studied in detail in this paper.
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| Maanen, M.A., Stoof, S.P., van der Zanden, E.P., de Jonge, W.J., Janssen, R.A., Fischer, D.F., Vandeghinste, N., Brys, R., Vervoordeldonk, M.J., Tak, P.P. (2009) The alpha7 nicotinic acetylcholine receptor on fibroblast-like synoviocytes and in synovial tissue from rheumatoid arthritis patients: A possible role for a key neurotransmitter in synovial inflammation. Arthritis Rheum. Vol. 60(5):1272-81.
Interleukin, IL8
Analytes tested using MSD assays: IL-8
Matrix tested: Tissue culture supernatants from Rheumatoid Arthritis FLS (Fibrobalst like synoviocytes collected and cultured from human synovial fluid)
Summary: The paper discusses the importance of a neurotransmitter (acetylcholine) in synovial inflammation and thus rheumatoid arthritis. Screening for cell surface expressed or secreted proteins was conducted that when down regulated can up-regulate proinflammatory cytokine expression.
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| Mak, G.J., Ledwidge, M.T., Watson, C.J., Phelan, D.M., Dawkins, I.R., Murphy, N.F., Patle, A.K., Baugh, J.A., McDonald, K.M. (2009) Natural History of Markers of Collagen Turnover in Patients With Early Diastolic Dysfunction and Impact of Eplerenone. J Am Coll Cardiol. Vol. 54(18):1674-82.
Interleukin, IL6, IL8, monocyte chemoattractant protein 1, C-reactive protein, tumor necrosis factor, TNFa, TNF-a, TNFalpha, TNFa, TNF-a
Analytes tested using MSD assays: IL-6, IL-8, TNF-alpha, MCP-1, CRP
Matrix tested: Human serum
Summary: This study assessed collagen turnover in preserved systolic function heart failure (HFPSF) patients and tested whether treatment with the aldosterone antagonist eplerenone has an impact on abnormalities in collagen metabolism in these patients.
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| Marco, A., Asa, D., Thomas, K., Lillemor, M., Olov, W., Bertil, W. (2009) Physical training after percutaneous coronary intervention in patients with stable angina: effects on working capacity, metabolism, and markers of inflammation. European Journal of Cardiovascular Prevention & Rehabilitation. [Epub in circulation].
Interleukin, IL8, IL6, IL10, c reactive protein
Analytes tested using MSD assays: C-reactive protein (CRP), serum amyloid A (SAA), IL-6, IL-8, IL-10
Matrix tested: Human serum
Summary: This study tested the hypothesis that exercise training improves glucose and lipid metabolism, the inflammatory / anti-inflammatory balance, and the outcome of elective percutaneous coronary intervention in patients with stable coronary disease.
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| Matt, P., Schoenhoff, F., Habashi, J., Holm, T., Van Erp, C., Loch, D., Carlson, O.D., Griswold, B.F., Fu, Q., Backer, J.D., PhD, Loey, B., Huso, D.L., McDonnell, N.B., Van Eyk, J.E., Dietz, H.C. (2009) Circulating Transforming Growth Factor-ß in Marfan Syndrome. Journal of American Heart Association. Vol. 120(6):526-32.
Transforming growth factor, TGFß1, TGF-ß1, TGFbeta
Analytes tested using MSD assays: TGF-beta1
Matrix tested: Human plasma
Summary: In this clinical study, the effects of a drug, losartan (blocks TGFß1 activation) on Marfan Syndrome were tested.
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| Mentor-Marcel, R.A., Bobe, G., Barrett, K.G., Young, M.R., Albert, P.S., Bennink, M.R., Lanza, E., Colburn, N.H. (2009) Inflammation-associated serum and colon markers as indicators of dietary attenuation of colon carcinogenesis in ob/ob mice. Cancer Prev Res. Vol. 2(1):60-9.
Interleukin, IL2, IL4, IL5, IL6, IL10, IL12, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, Granulocyte Macrophage Colony Stimulating Factor, Monocyte Chemotactic Protein
Analytes tested using MSD assays: Mouse Th1/Th2 9-Plex (IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, KC / GRO / CINC, IL-10, IL-12, TNF-alpha), Mouse Custom Metabolic Panel (IL-6, GM-CSF, Insulin, MCP-1, Resistin)
Matrix tested: Mouse serum
Summary: This paper is based on the study of changes in specific obesity or inflammation related proteins and the corresponding genes in colon mucosa as a result of modification of diet.
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| Patel, A., Murray, J., McElwee-Whitmer, S., Bai, C., Kunapuli, P., Johnson, E.N. (2009) A combination of ultrahigh throughput PathHunter and cytokine secretion assays to identify glucocorticoid receptor agonists. Anal Biochem. Vol. 385(2):286-92.
Interleukin, IL6, IL8, tumor necrosis factor, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: IL-1beta, IL-6, IL-8, TNF-alpha
Matrix tested: Cell culture supernatants
Summary: In this study, Path Hunter technology (a registered trademark of DiscoveRx Corporation) combined with cytokine secretion assays were used to screen an entire Merck compound library for glucocorticoid receptor (GR) agonists in a 3456-well plate format.
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| Patel, K.D., Duggan, S.P., Currid, C.A., Gallagher, W.M., McManus, R., Kelleher, D., Murphy, R.T., Ryan, A.W. (2009) High sensitivity cytokine detection in acute coronary syndrome reveals up-regulation of Interferon Gamma and Interleukin-10 post Myocardial Infarction. Clinical Immunology. Vol. 133(2):251-6.
Interleukin, IL2, IL6, IL8, IL10, IL12p70, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, tumor necrosis factor, Granulocyte Macrophage Colony Stimulating Factor, Monocyte Chemotactic Protein
Analytes tested using MSD assays: Human ProInflammatory 9-Plex Ultra-Sensitive Kit (GM-CSF, IFN-gamma, IL-1beta, IL-10, IL-12 p70, IL-2, IL-6, IL-8, TNF-alpha)
Matrix tested: Human serum
Summary: This is a clinical study to determine specific cytokines that are upregulated in myocardial infarction and can therefore serve as biomarkers for the same.
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| Prencipe, M., Fitzpatrick, P., Gorman, S., Tosetto, M., Klinger, R., Furlong, F., Harrison, M., O'Connor, D., Roninson, I.B., O'Sullivan, J., McCann, A. (2009) Cellular senescence induced by aberrant MAD2 levels impacts on paclitaxel responsiveness in vitro. Br J Cancer. Vol. 101(11):1900-8.
Interleukin, IL6, IL8, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, mitotic arrest deficiency protein, chemotherapy
Analytes tested using MSD assays: IL-1beta, IL-6, IL-8
Matrix tested: MCF7 cell culture supernatants
Summary: In this paper, the role of MAD2 protein levels have been studied in association with developing resistance to a chemotherapeutic drug called paclitaxel.
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| Redd, A.D., Dabitao, D., Bream, J.H., Charvat, B., Laeyendecker, O., Kiwanuka, N., Lutalo, T., Kigozi, G., Tobian, A.A., Gamiel, J., Neal, J.D., Oliver, A.E., Margolick, J.B., Sewankambo, N., Reynolds, S.J., Wawer, M.J., Serwadda, D., Gray, R.H., Quinn, T.C. (2009) Microbial translocation, the innate cytokine response, and HIV-1 disease progression in Africa. Proc Natl Acad Sci U S A. Vol. 106(16):6718-23.
Interleukin, IL2, IL6, IL8, IL10, IL12p70, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, tumor necrosis factor, granulocyte macrophage colony stimulating factor
Analytes tested using MSD assays: GM-CSF, IFN-gamma, IL-1beta, IL-2, IL-6, IL-8, IL-10, IL-12p70, TNF-alpha
Matrix tested: Human serum
Summary: This study explored the longitudinal relationship between microbial translocation, immune activation and circulating inflammatory cytokine response in a cohort of HIV-1 infected individuals with different rates of disease progression.
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| Remondo, C., Cereda, V., Mostbock, S., Sabzevari, H., Franzusoff, A., Schlom, J., Tsang, K.Y. (2009) Human dendritic cell maturation and activation by a heat-killed recombinant yeast (Saccharomyces cerevisiae) vector encoding carcinoembryonic antigen. Vaccine. Vol. 27(7):987-94.
Interleukin, IL2, IL4, IL5, IL8, IL10, IL12p70, IL13, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, tumor necrosis factor
Analytes tested using MSD assays: IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, IL-8, IL-10, IL-12p70, IL-13, TNF-alpha
Matrix tested: Human dendritic cell culture supernatants
Summary: This study examines the use of a yeast vector containing the transgene for human carcinoembryonic antigen (CEA) in cancer immunotherapy due to its ability to activate human dendritic cells (DCs) and stimulate CEA-specific T-cell responses.
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| Russell, F.A., Fernandesa, E.S., Couradeb J.P., Keeblea, J.E., Brain, S.D. (2009) Tumour necrosis factor a mediates transient receptor potential vanilloid 1-dependent bilateral thermal hyperalgesia with distinct peripheral roles of interleukin-1ß, protein kinase C and cyclooxygenase-2 signaling. Pain. Vol. 142(3):264-274.
Interleukin, IL6, IL10, IL12, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, tumor necrosis factor
Analytes tested using MSD assays: IL-1beta, IL-6, IL-10, IL-12, IFN-gamma, KC / GRO / CINC, TNF-alpha
Matrix tested: Mouse paw homogenates
Summary: In this study, the mechanisms that link the inflammatory and nociceptive effects of TNF-alpha have been examined.
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Russo, A.L., Jedlicka, K., Wernick, M., McNally, D., Kirk, M., Sproull, M., Smith, S., Shankavaram, U., Kaushal, A., Figg, W.D., Dahut, W., Citrin, D., Bottaro, D.P., Albert, P.S., Tofilon, P.J., Camphausen, K. (2009) Urine Analysis and Protein Networking Identify Met as a Marker of Metastatic Prostate Cancer. Clin Cancer Res. Vol. 15(13):4292-4298.
Analytes tested using MSD assays: Met
Matrix tested: Urine
Summary: This study is based on the validation of Met as a marker for metastatic prostrate cancer since its levels differ significantly between localized disease group and the metastatic group.
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| Sears, D.D., Miles, P.D., Chapman, J., Ofrecio, J.M., Almazan, F., Thapar, D., Miller, Y.I. (2009) 12/15-Lipoxygenase Is Required for the Early Onset of High Fat Diet-Induced Adipose Tissue Inflammation and Insulin Resistance in Mice. PLoS One. Vol. 4 (9):7250.
Interleukin, IL6, IL10, IL12p70, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, tumor necrosis factor
Analytes tested using MSD assays: IFN-gamma, IL-1beta, IL-6, IL-10, IL-12p70, KC / GRO / CINC, TNF-alpha
Matrix tested: Mouse epididymal white adipose tissue (eWAT) lysates and plasma
Summary: In this paper, the authors tested whether 12/15-Lipoxygenase gene product is associated with high fat diet induced insulin resistance in mice.
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| Siddiqui, S., Cruse, G., McKenna, S., Monteiro, W., Mistry, V., Wardlaw, A., Brightling, C. (2009) IL-13 expression by blood T cells and not eosinophils is increased in asthma compared to non-asthmatic eosinophilic bronchitis. BMC Pulmonary Medicine. Vol. 9:34.
Interleukin, IL2, IL4, IL5, IL8, IL10, IL12p70, IL13, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, IL8, Monocyte Chemotactic Protein, Macrophage-Derived Chemokine, Thymus and Activation Regulated Chemokine, Interferon Inducible Protein 10, MIP1ß, MIP1b, MIP1beta
Analytes tested using MSD assays: Human TH1/TH2 10-Plex (IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, IL-8, IL-10, IL-12p70, IL-13, TNF-alpha), Human Chemokine 9-Plex (Eotaxin, Eotaxin-3, IL-8, IP-10, MCP-1, MCP-4, MDC, MIP-1beta, TARC)
Matrix tested: Human serum
Summary: This study is based on the comparison of IL-13 expression in peripheral blood T-cells and eosinophils from asthma patients with healthy controls and attempts to identify an inflammatory stimulus for this expression.
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| Singh, B.N., Hayes, G.R., Lucas, J.J., Sommer, U., Viseux, N., Mirgorodskaya, E., Trifonova, R.T., Sassi, R.R., Costello, C.E., Fichorova, R.N. (2009) Structural details and composition of Trichomonas vaginalis lipophosphoglycan in relevance to the epithelial immune function. Glycoconj J. Vol. 26(1):3-17.
Interleukin, IL8, ERK, NF kappa B, MAPK, Vaginal mucosal immunity
Analytes tested using MSD assays: IL-8, Phospho ERK1/2, Phospho NF-kappaB/p65 (Ser538), Phospho MEK
Matrix tested: Human epithelial cell culture supernatants
Summary: This study examines the biochemical nature of Trichomonas vaginalis surface lipophosphoglycan (LPG) and determines the molecular domains necessary for host immunoinflammatory response.
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| Sjöberg, S., Eriksson, E.E., Tivesten, A., Carlsson, A., Klasson, A., Levin, M., Borén, J., Krettek, A. (2009) CD44-deficiency on hematopoietic cells limits T-cell number but does not protect against atherogenesis in LDL receptor-deficient mice. Atherosclerosis. Vol. 206(2):369-74.
Interleukin, IL2, IL4, IL5, IL8, IL10, IL12, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: Mouse Th1/Th2 9-Plex (IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, KC / GRO / CINC, IL-10, IL-12, TNF-alpha)
Matrix tested: Mouse cell culture supernatants
Summary: In this study, bone marrow reconstitution experiments were done in low density lipoprotein (LDL) receptor deficient mouse model to test whether CD44 expressed by hematopoietic cells plays a critical role in the pathology of atherogenesis.
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| Symonds, E.L., Riedel, C.U., O'Mahony, D., Lapthorne, S., O'Mahony, L., Shanahan, F. (2009) Involvement of T helper type 17 and regulatory T cell activity in Citrobacter rodentium invasion and inflammatory damage. Clinical & Experimental Immunology. Vol. 157(1):148-154.
Interleukin, IL6, IL10, IL12p70, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, IL8, tumor necrosis factor
Analytes tested using MSD assays: IFN-gamma, IL-1beta, IL-6, IL-10, IL-12p70, KC / GRO / CXCL1, TNF-alpha
Matrix tested: Mouse cell culture supernatants
Summary: In this paper, the main signaling events associated with the clearance of Citrobacter rodentium (induces colitis) from the colon have been studied in mice.
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| Trifonova, R.T., Doncel, G.F., Fichorova, R.N. (2009) Polyanionic Microbicides Modify TLR_mediated Cervicovaginal immune response. Antimicrob Agents Chemother. Vol. 53(4):1490-500.
Interleukin, Interferon, IL8, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, IFNb, IFB-b, IFNbeta, IFN-ß, IFNß, HIV-1
Analytes tested using MSD assays: IL-8, IL-1beta, IFN-beta
Matrix tested: Human cervical and vaginal epithelial cell culture supernatants and lysates
Summary: This study tests the effects of anti-HIV microbicides dextran sulfate and polystyrene sulfonate on Toll-like receptor signaling in human epithelial cell lines.
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| Urry, Z., Xystrakis, E., Richards, D.F., McDonald, J., Sattar, Z., Cousins, D.J., Corrigan, C.J., Hickman, E., Brown, Z., Hawrylowicz, C.M. (2009) Ligation of TLR9 induced on human IL-10-secreting Tregs by 1alpha,25-dihydroxyvitamin D3 abrogates regulatory function. J Clin Invest. Vol. 119(2):387-98.
Interleukin, IL5, IL10, IL13, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g
Analytes tested using MSD assays: IL-5, IL-10, IL-13, IFN-gamma
Matrix tested: Human peripheral blood mononuclear cell (PBMC) supernatants
Summary: In this paper, the effect of 1a25VitD3 (the active form of Vitamin D) on IL-10 producing Treg cells and Toll like receptor signaling has been discussed.
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| Watson, J.A., Watson, C.J., McCrohan, A.M., Woodfine, K., Tosetto, M., McDaid, J., Gallagher, E., Betts, D., Baugh, J., O'Sullivan, J., Murrell, A., Watson, R.W., McCann, A. (2009) Generation of an epigenetic signature by chronic hypoxia in prostate cells. Human Molecular Genetics. Vol. 18(19):3594-604.
Interleukin, IL8, IL6, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: IL-1beta, IL-6, IL-8 and TNF-alpha
Matrix tested: Tissue culture supernatants
Summary: In this study, a benign prostrate cancer cell line has been compared to a hypoxic-conditioned prostrate cancer cell line to identify phenotypic and epigenetic consequences of chronic hypoxia in relation to cancer, apoptosis, invasion and cellular senescence.
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| Wigren, M., Bengtsson, D., Dunér, P., Olofsson, K., Björkbacka, H., Bengtsson, E., Fredrikson, G.N., Nilsson, J. (2009) Atheroprotective Effects of Alum Are Associated With Capture of Oxidized LDL Antigens and Activation of Regulatory T Cells. Circ Res. Vol. 104(12):62-70.
Interleukin, IL2, IL4, IL5, IL10, IL12, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: Mouse TH1/TH2 9-Plex (IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, IL-10, IL-12, KC / GRO / CINC, TNF-alpha)
Matrix tested: Mouse splenocyte cell culture supernatants
Summary: In this paper, the mechanism for anti-atherogenic properties of alum has been investigated by administering alum in the absence of an antigen into mice.
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| Willett, C.G., Duda, D.G., di Tomaso, E., Boucher, Y., Ancukiewicz, M., Sahani, D.V., Lahdenranta, J., Chung, D.C., Fischman, A.J., Lauwers, G.Y., Shellito, P., Czito, B.G., Wong, T.Z., Paulson, E., Poleski, M., Vujaskovic, Z., Bentley, R., Chen, H.X., Clark, J.W., Jain, R.K. (2009) Efficacy, Safety, and Biomarkers of Neoadjuvant Bevacizumab, Radiation Therapy, and Fluorouracil in Rectal Cancer: A Multidisciplinary Phase II Study. Journal of Clinical Oncology. Vol. 27(18):3020-3026.
Vascular endothelial growth factor receptor, Placental growth factor, Basic fibroblast growth factor, Granulocyte macrophage colony-stimulating factor, Interleukin, IL6, IL8, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, tumor necrosis factor
Analytes tested using MSD assays: soluble VEGFR, VEGF, PlGF, bFGF, GM-CSF, IL-1beta, IL-6, IL-8, and TNF-alpha
Matrix tested: Human EDTA plasma
Summary: This is a phase II clinical study determining the effects of Bevacizumab treatment on localized rectal cancer.
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| Xu, Y., Li, J., Ferguson, G.D., Mercurio, F., Khambatta, G., Morrison, L. Lopez-Girona, A., Corral, L.G., Webb, D.R., Bennett, B.L., Xie, W. (2009) Immunomodulatory drugs reorganize cytoskeleton by modulating Rho GTPases. Blood. Vol. 114(2):338-345.
Interleukin, IL2
Analytes tested using MSD assays: Human IL-2
Matrix tested: Human peripheral blood mononuclear cell (PBMC) culture supernatants
Summary: In this paper, the mechanism of action of immunomodulatory drugs has been investigated.
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| Yang, J.B., James, E., Roti, M., Huston, L., Gebe, J.A., Kwok, W.W. (2009) Searching immunodominant epitopes prior to epidemic: HLA class II-restricted SARS-CoV spike protein epitopes in unexposed individuals. Intrnl Immuno. Vol. 21(1):63-71.
Interleukin, IL5, IL10, IL13, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g
Analytes tested using MSD assays: IFN-gamma, IL-5, IL-10, IL-13
Matrix tested: Human serum
Summary: In this study, the authors have developed a novel tetramer guided epitope mapping (TGEM) method to identify dominant CD4+ T-cell epitopes in SARS virus from subjects that have not been exposed to the pathogen.
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| Yamasaki, S, Matsumoto, M., Takeuchi, O., Matsuzawa, T., Ishikawa, E., Sakuma, M., Tateno, H., Uno, J., Hirabayashi, J., Mikami, Y., Takeda, K., Akira, S., Saito, T. (2009) C-type lectin Mincle is an activating receptor for pathogenic fungus, Malassezia. Proc Natl Acad Sci USA. Vol. 106(6):1897-902.
ITAM, TNF, IL-10, KC / GRO / CINC
Analytes tested using MSD assays: Cytokines (specific cytokines not mentioned)
Matrix tested: Malassezia activated macrophage culture
Summary: In this study, the role of Mincle receptor in eliciting an immune response to Malassezia fungal infections has been examined.
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| Zhu, A.X., Sahani, D.V., Duda, D.G., di Tomaso, E., Ancukiewicz, M., Catalano, O.A., Sindhwani, V., Blaszkowsky, L.S. Yoon, S.S., Lahdenranta, J., Bhargava, P., Meyerhardt, J. Clark, J.W., Kwak, E.L., Hezel, A.F., Miksad, R., Abrams, T.A., Enzinger, P.C., Fuchs, C.S., Ryan, D.P., Jain, R.K. (2009) Efficacy, Safety, and Potential Biomarkers of Sunitinib Monotherapy in Advanced Hepatocellular Carcinoma: A Phase II Study. Journal of Clinical Oncology. Vol. 27(18):3027-3035.
Interleukin, IL6, IL8, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, Vascular endothelial growth factor receptor, Placental growth factor, Basic fibroblast growth factor
Analytes tested using MSD assays: VEGF, PlGF, soluble VEGFR1, bFGF, IL-1beta, IL-6, IL-8, TNF-alpha
Matrix tested: Human EDTA plasma
Summary: This paper discusses the results of a multidisciplinary clinical trial using sunitinib drug to treat hepatocellular carcinoma.
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| Calvo, K.R., Dabir, B., Kovach, A., Devor, C., Bandle, R., Bond, A., Shih, J.H., Jaffe, E.S. (2008) IL-4 protein expression and basal activation of Erk in vivo in follicular lymphoma. Blood. Vol. 112(9):3818-26.
Interleukin, IL2, IL4, IL5, IL8, IL10, IL12p70, IL13, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, IL-8, IL-10, IL-12p70, IL-13, TNF-alpha
Matrix tested: Human lymph node cell lysates from follicular lymphoma patients
Summary: In this paper, the cytokine profile in lymph nodes of patients with Follicular Lymphoma was studied to better understand signaling networks associated with tumor.
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| Catalfamo, M., Di Mascio, M., Hu, Z., Srinivasula, S., Thaker, V., Adelsberger, J., Rupert, A., Baseler, M., Tagaya, Y., Roby, G., Rehm, C., Follmann, D., Lane, H.C. (2008) HIV infection-associated immune activation occurs by two distinct pathways that differentially affect CD4 and CD8 T cells. PNAS. Vol. 105(50):19851-19856.
Interleukin, IL2, IL4, IL5, IL8, IL10, IL12p70, IL13, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, IL-8, IL-10, IL-12p70, IL-13, TNF-alpha
Matrix tested: Human serum
Summary: The mechanism of immune activation and CD4 depletion during HIV infection has been discussed in this paper.
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| de la Rosa, G., Yang, D., Tewary, P., Varadhachary, A., Oppenheim, J.J. (2008) Lactoferrin acts as an alarmin to promote the recruitment and activation of APCs and antigen-specific immune responses. J Immunol. Vol. 180(10):6868-76.
Interleukin, IL2, IL4, IL5, IL8, IL10, IL12p70, IL13, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: Human TH1/TH2 10-Plex (IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, IL-8, IL-10, IL-12p70, IL-13, TNF-alpha
Matrix tested: Human peripheral blood mononuclear cell (PBMC) culture supernatants
Summary: In this paper, the interaction of lactoferrin with cells of the immune system has been investigated in detail.
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| Dennis, A., Kudo, T., Kruidenier, L., Girard, F., Crepin, V.F., MacDonald, T.T., Frankel, G., Wiles, S. (2008) The p50 subunit of NF-kappaB is critical for in vivo clearance of the noninvasive enteric pathogen Citrobacter rodentium. Infect Immun. Vol. 76(11):4978-88.
Interleukin, IL6, IL10, IL12, IL12-p70, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, KC / GRO / CINC, tumor necrosis factor, NFkB, Nuclear Factor kappa B
Analytes tested using MSD assays: Mouse ProInflammatory 7-plex (IFN-gamma, IL-1beta, IL-6, IL-10, IL-12p70, KC/GRO/CINC, TNF-alpha
Matrix tested: Mouse colon tissue homogenates
Summary: The transcription factor, NF-kB, has been found to be crucial in maintaining intestinal inflammation during host defense, and in this study, the immune response of mice lacking the p50 subunit of NF-kB during C. rodentium infection has been assessed.
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| Dillon, T.M., Ricci, M.S., Vezina, C., Flynn, G.C. Liu, Y. D., Rehder, D.S., Plant, M., Henkle, B., Li, Y., Deechongkit, S., Varnum, B., Wypych, J., Balland, A., Bondarenko P. V. (2008) Structural and functional characterization of disulfide isoforms of the human IGG2 subclass. J Biol Chem. Vol. 283(23):16206-15.
Interleukin, IL6
Analytes tested using MSD assays: Human IL-6
Matrix tested: Human serum
Summary: In this paper, a method has been described to make disulphide forms of human IgG2 antibody and the potency and biophysical properties of these forms have been discussed.
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| Dower, K., Ellis, D.K., Saraf, K, Jelinsky, S.A., Lin, L.L. (2008) Innate Immune Responses to TREM-1 Activation: Overlap, Divergence, and Positive and Negative Cross-Talk with Bacterial Lipopolysaccharide. J Immunol. Vol. 180(5):3520-34.
Interleukin, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, IL12p40, IL12-p40, Granulocyte Macrophage Colony Stimulating Factor
Analytes tested using MSD assays: IL-1beta, IL-12p40, GM-CSF
Matrix tested: Cell culture supernatants
Summary: In this paper, the cellular consequences of TREM-1 activation have been investigated by characterizing global gene expression changes in human monocytes in response to TREM-1 cross-linking in comparison to and combined with LPS.
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| Fichorova R.N., Richardson-Harman, N., Alfano, M., Belec, L., Carbonneil, C., Chen, S., Cosentino, L., Curtis, K., Dezzutti, C.S., Donoval, B. Doncel, G.F., Donaghay, M., Grivel, J.C., Guzman, E., Hayes, M., Herold, B, Hillier, S., Lackman-Smith, C., Landay, A., Margolis, L., Mayer, K.H., Pasicznyk, J.M., Pallansch-Cokonis, M., Poli, G., Reichelderfer, P., Roberts, P., Rodriguez, I., Saidi, H., Sassi, R.R., Shattock, R., Cummins, J.E. Jr. (2008) Biological and technical variables affecting immunoassay recovery of cytokines from human serum and simulated vaginal fluid: a multicenter study. Anal Chem. Vol. 80(12): 4741-51.
Interleukin, IL6, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: Human IL-1beta, IL-6
Matrix tested: Human serum and surrogate vaginal fluid
Summary: In this paper, different immunoassay formats (microplate vs bead-based) from different companies were evaluated to measure cytokines in complex biological fluids such as surrogate vaginal fluid and human serum. Intra- and Interlaboratory comparisons were done by 12 laboratories in four countries, using 14 immunoassays and four detection platforms based on absorbance, chemiluminescence, electrochemiluminescence, and fluorescence.
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| Fitzgerald, P., Cassidy Eugene, M., Clarke, G., Scully, P., Barry, S., Quigley Eamonn, M.M., Shanahan, F., Cryan, J., Dinan, T. G. (2008) Tryptophan catabolism in females with irritable bowel syndrome: relationship to interferon-gamma, severity of symptoms and psychiatric co-morbidity. Neurogastroenterol Motil. Vol. 20(12):1291-7.
Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g
Analytes tested using MSD assays: IFN-gamma
Matrix tested: Human plasma
Summary: This study examined the hypothesis that Irritable bowel syndrome is associated with increased tryptophan (Trp) catabolism along the Kynurenine pathway due to increased IFN-gamma levels.
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| Grund, E.M., Kagan, D., Tran, C.A., Zeitvogel, A., Starzinski-Powitz, A., Nataraja, S., Palmer, S.S. (2008) Tumor necrosis factor-alpha regulates inflammatory and mesenchymal responses via mitogen-activated protein kinase kinase, p38, and nuclear factor kappaB in human endometriotic epithelial cells. Mol Pharmacol. Vol. 73(5):1394-404.
Interleukin, IL6, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: IL-6, IL-8, GMCSF, MCP-1, phospho (Thr180/Tyr182) and total p38, phospho (Ser473) and total Akt, phospho NFkB (Ser468), phospho (Thr202/Tyr204) and total Erk
Matrix tested: Cell culture supernatants
Summary: This study explores the ability of TNF-alpha binding protein (TBP) and kinase inhibitors to reduce the inflammatory and necrotic effects of TNF-alpha in relation to endometriosis.
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| Haskova, Z., Hoang, B., Luo, G., Morgan, L.A., Billin, A.N., Barone, F.C., Shearer, B.G., Barton, M.E., Kilgore, K.S. (2008) Modulation of LPS-induced pulmonary neutrophil infiltration and cytokine production by the selective PPARbeta/delta ligand GW0742. Inflamm Res. Vol. 57(7):314-21.
Interleukin, IL6, IL8, Monocyte Chemotactic Protein, Macrophage-Derived Chemokine, Granulocyte Macrophage Colony Stimulating Factor
Analytes tested using MSD assays: IL-1beta, IL-6, TNF-alpha
Matrix tested: Mouse bronchoalveolar lavage fluid (BALF)
Summary: This study explored the anti-inflammatory effects of PPARß/d activation by use of the selective PPARß/d ligand (GW0742) in a mouse model of lipopolysaccharide (LPS)-induced pulmonary inflammation.
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| Herberich, B., Cao, G.Q., Chakrabarti, .PP., Falsey, J.R., Pettus, L., Rzasa, R.M., Reed, A.B., Reichelt, A., Sham, K., Thaman, M., Wurz, R.P., Xu, S., Zhang, D., Hsieh, F., Lee, M.R., Syed, R., Li, V., Grosfeld, D., Plant, M.H., Henkle, B., Sherman, L., Middleton, S., Wong, L.M., Tasker, A.S. (2008) Discovery of Highly Selective and Potent p38 Inhibitors Based on a Phthalazine Scaffold. J Med Chem. Vol. 51 (20):6271–6279.
Interleukin, IL8, tumor necrosis factor, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, quinazoline, SAR
Analytes tested using MSD assays: IL-8, TNF-alpha
Matrix tested: Cell culture supernatant, rat plasma
Summary: In this study, novel phthalazine-based inhibitors of p38 have been generated and their structure-activity relationship has been examined to make orally bioavailable inhibitors with good pharmacokinetic properties for the treatment of inflammatory diseases.
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| Inglefield, J.R., Dumitru, C.D., Alkan, S.S., Gibson, S.J., Lipson, K.E., Tomai, M.A., Larson, C.J., Vasilakos, J.P. (2008) TLR7 Agonist 852A Inhibition of Tumor Cell Proliferation Is Dependent on Plasmacytoid Dendritic Cells and Type I IFN. J Interferon Cytokine Res. Vol. 28(4):253-63.
Interleukin, IL8, tumor necrosis factor, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, quinazoline, SAR
Analytes tested using MSD assays: MCP-1, IP-10, IL-12p70
Matrix tested: Human peripheral blood mononuclear cell (PBMC) culture supernatants
Summary: The antitumor effects of a TLR7 agonist, 852A, has been evaluated in this paper by measuring the .ability of 852A induced cytokines to block in vitro and in vivo tumor proliferation.
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| Koppelman, B., Webb, H.K., Medicherla, S., Almirez, R., Feng, Y., Chavez, J.C., Mao, C.P., Nguyen, A., Liu, Y.W., Kapoun, A.M., Muiru, G., Huang, Y.A., Dugar, S., Mavunkel, B.J., Lim, D.W., Chakravarty, S., Luedtke, G., Protter, A.A., Higgins, L.S. (2008) Pharmacological properties of SD-282 - an alpha-isoform selective inhibitor for p38 MAP kinase. Pharmacology. Vol. 81(3):204-20.
Interleukin, IL6, IL8, tumor necrosis factor, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, p38 MAP kinase, Asthma, sepsis
Analytes tested using MSD assays: IL-1beta, IL-6, IL-8, TNF-alpha
Matrix tested: Human plasma
Summary: The focus of this study was to examine the pharmacological properties of SD-282, which displays a very high specificity for inhibiting p38 MAPK, on cytokine production in vitro as well as in vivo.
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| Kwok, W.W., Yang, J., James, E., Bui, J., Huston, L., Wiesen, A.R., Roti, M. (2008) The anthrax vaccine adsorbed vaccine generates protective antigen (PA)-Specific CD4+ T cells with a phenotype distinct from that of naive PA T cells. Infect Immun. Vol. 76(10):4538-45.
Interleukin, IL5, IL13, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g
Analytes tested using MSD assays: IFN-gamma, IL-5, IL-13
Matrix tested: Cell culture supernatants
Summary: In this paper, a group of individuals immunized with anthrax vaccine was compared with a group of unvaccinated volunteers to examine the CD4+ T-cell responses against Protective Antigen elicited by vaccination.
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| Lee, J.K., McCoy M.K, Harms, A.S, Ruhn, K.A., Gold S.J., Tansey M.G. (2008) Regulator of G-protein signaling 10 promotes dopaminergic neuron survival via regulation of the microglial inflammatory response. J Neurosci. Vol. 28(34):8517-28.
Interleukin, IL6, IL10, IL12, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, tumor necrosis factor, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: IFN-gamma, IL-1beta, IL-6, IL-10, IL-12, KC / GRO, TNF-alpha
Matrix tested: Mouse microglial BV2 cell culture supernatants
Summary: This paper discusses the regulatory effect of RGS10 (regulator of G-protein signaling 10) on microglial activation and neuron survival in relation to Parkinson’s disease.
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| Leng, S.X., McElhaney, J.E., Walston J.D., Xie D.X., Fedarko N.S., Kuchel, G.A. (2008) ELISA and Multiplex Technologies for Cytokine Measurement in Inflammation and Aging Research. J Gerontol A Biol Sci Med Sci. Vol. 63(8):879-84.
MESO SCALE DISCOVERY, Luminex, ELISA, multiplex, aging, inflammation, multiplex arrays
Summary: This paper compares ELISA with different multiplex technologies with respect to technical aspects, cost and methodological issues for the measurement of cytokines in older adults.
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| Li, Y.Y., Bao, M., Meurer, J., Skuballa, W., Bauman, J.G., Doecke, W.D., Zollner, T.M. (2008) The identification of a small molecule inhibitor that specifically reduces T cell-mediated adaptive but not LPS-mediated innate immunity by T cell membrane-monocyte contact bioassay. Immunol Lett. Vol. 117(1):114-8.
384-well assay, tumor necrosis factor, TNFa, TNF-a, TNFalpha, TNFa, TNF-a
Analytes tested using MSD assays: TNF-alpha
Matrix tested: Human peripheral blood mononuclear cell (PBMC) culture supernatants
Summary: In this study, the authors developed a T cell-monocyte contact bioassay to identify compounds that block TNF-alpha production from T cell-mediated, but not LPS-mediated, monocyte activation, and will therefore have an improved side-effect profile.
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Lloyd, E., Somera-Molina, K., Van Eldik, L.J., Watterson, D.M., Wainwright, M.S. (2008) Suppression of acute proinflammatory cytokine and chemokine upregulation by post-injury administration of a novel small molecule improves long-term neurologic outcome in a mouse model of traumatic brain injury.
J Neuroinflammation. Vol. 30(5):28.
Interleukin, IL-1beta, IL-1b, IL1ß, IL1b, IL6, TNF-a, TNFa, TNFa, TNF-a, TNFalpha
Analytes tested using MSD assays: IL-1ß, IL-6, TNF-alpha and CCL2
Matrix tested: Brain extracts from mouse model of closed head injury
Summary: This paper tested the hypothesis that regulation of cytokine and chemokine levels following traumatic brain injury causes a decrease in neurologic injury and improves brain function. A small molecule experimental drug called Minozac was used to suppress the up-regulation of cytokines after injury.
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| Luger, D., Silver, P.B., Tang, J., Cua, D., Chen, Z., Iwakura, Y., Bowman, E.P., Sgambellone, N.M., Chan, C.C., Caspi, R.R. (2008) Either a Th17 or a Th1 effector response can drive autoimmunity: conditions of disease induction affect dominant effector category. J Exp Med. Vol. 205(4):799-810.
Interleukin, IL17
Analytes tested using MSD assays: Customer developed IL-17F assay
Matrix tested: Cell culture supernatants
Summary: In this paper, the role of IL-23 – IL-17 pathway in the pathogenesis of experimental autoimmune uveitis has been examined.
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| Luqman, M., Klabunde, S., Lin, K., Georgakis, G.V., Cherukuri, A., Holash, J., Goldbeck, C., Xu, X., Kadel, E.E. 3rd, Lee, S.H., Aukerman, S.L., Jallal, B., Aziz, N., Weng, W.K., Wierda, W., O'Brien, S., Younes, A. (2008) The antileukemia activity of a human anti-CD40 antagonist antibody, HCD122, on human chronic lymphocytic leukemia cells. Blood. Vol. (112)3:711-720.
Interleukin, IL6, IL8, IL10, tumor necrosis factor, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, Granulocyte Macrophage Colony Stimulating Factor
Analytes tested using MSD assays: IL-6, IL-8, IL-10, GM-CSF, TNF-alpha
Matrix tested: Human peripheral blood mononuclear cell (PBMC) culture supernatants
Summary: This study demonstrates the effect of HCD122, a novel anti-CD40 monoclonal antibody, in inhibiting CD40 ligand-induced activation of signaling pathways, proliferation and survival of B-cell chronic lymphocytic leukemia cells.
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| Mathieu, M.C., Lord-Dufour, S., Bernier, V., Boie, Y., Burch, J.D., Clark, P., Denis, D., Han, Y., Mortimer, J.R., Therien, A.G. (2008) Mutual antagonistic relationship between prostaglandin E(2) and IFN-gamma: Implications for rheumatoid arthritis. Eur J Immunol. Vol. 38(7):1900-12.
Interleukin, IL12, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, Interferon Inducible Protein 10
Analytes tested using MSD assays: IFN-gamma, IL-12, IP-10
Matrix tested: Human plasma, human fibroblast-like synoviocytes (FLS) cell culture supernatants
Summary: In this paper, the roles of prostaglandin E2 and IFN-gamma in chronic inflammation associated with rheumatoid arthritis has been investigated.
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| McDaniel, J.C., Belury, M., Ahijevych, K., Blakely, W. (2008) Omega-3 fatty acids effect on wound healing. Wound Repair Regen. Vol. 16(3):337-45.
Interleukin, IL6, tumor necrosis factor, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: IL-1 beta, IL-6, TNF-alpha
Matrix tested: Wound blister fluid
Summary: In this paper, the effects of marine-derived o-3 eicosapentaenoic (EPA) and docosahexaenoic (DHA) polyunsaturated fatty acids on the levels of proinflammatory cytokines in wound fluid and the time to complete healing in healthy, human skin were evaluated.
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| Netterwald, J. (2008) Immuno’s Logical - Drug developers turn to the new and established immunoassay platforms to measure and identify protein biomarkers. Drug Disc Devel. Vol. 11(10):24-28.
Interleukin, IL6, tumor necrosis factor, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Summary: This is a magazine article that serves as a minireview of newly available immunoassay platforms for the identification of protein biomarkers.
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| Newland, N., Richter, A. (2008) Agents associated with lung inflammation induce similar responses in NCI-H292 lung epithelial cells. Toxicol In Vitro. Vol. 22(7):1782-8.
Interleukin, IL6, IL8
Analytes tested using MSD assays: IL-6, IL-8
Matrix tested: Cell culture supernatant
Summary: In this study, the authors examined the ability of a lung epithelial model to respond to lung toxicants including cigarette smoke total particulate matter, fly ash, bleomycin, lipopolysaccharide, vanadyl sulphate, diesel exhaust particles, and carbon black.
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| Patsouris, D, Li, P.P., Thapar, D., Chapman, J., Olefsky, J.M., Neels, J.G. (2008) Ablation of CD11c-positive cells normalizes insulin sensitivity in obese insulin resistant animals. Cell Metab. Vol. 8(4):301-9.
Interleukin, IL6, IL10, IL12p70, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, Monocyte Chemotactic Protein, Granulocyte Macrophage Colony Stimulating Factor
Analytes tested using MSD assays: Mouse pro-inflammatory 7 plex (IFN-gamma, IL-1beta, IL-6, IL-10, IL-12p70, KC / GRO / CINC, TNF-alpha), Custom Metabolic panel (IL-6, GM-CSF, insulin, MCP-1, resistin, TNF-alpha)
Matrix tested: Mouse serum and tissue lysates
Summary: This study investigates the role of a specific subset of macrophages that express CD11c in the development of obesity-associated insulin resistance and type II diabetes.
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| Roti, M., Yang, J., Berger, D., Huston, L., James, E.A., Kwok, W.W. (2008) Healthy human subjects have CD4+ T cells directed against H5N1 influenza virus. J Immunol. Vol. 180(3):1758-68.
Interleukin, IL5, IL13, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor
Analytes tested using MSD assays: IFN-gamma, IL-5, IL-13, TNF-alpha
Matrix tested: Human peripheral blood mononuclear cell (PBMC) culture supernatants
Summary: This study tested the hypothesis that CD4+ T cells generated in response to other Influenza A viruses can recognize H5N1 viral proteins due to homology between the viral proteins of different influenza A subtypes.
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| Ryan, K.R., Hong, M., Arkwright, P.D., Gennery, A.R., Costigan, C., Dominguez, M., Denning, D., McConnell, V., Cant, A.J., Abinun, M., Spickett, G.P., Lilic, D. (2008) Impaired dendritic cell maturation and cytokine production in patients with chronic mucocutanous candidiasis with or without APECED. Clin Exp Immunol. Vol. 154(3):406-14.
Interleukin, IL2, IL5, IL6, IL10, IL12p70, IL13, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor
Analytes tested using MSD assays: IFN-gamma, IL-2, IL-5, IL-6, IL-10, IL-12p70, IL-13, TNF-alpha
Matrix tested: Human monocyte-derived dendritic cell (moDC) culture supernatants
Summary: This study investigated dendritic cell function in response to Candida and non-Candida infections, to assess the underlying mechanisms in the pathogenicity of chronic mucocutaneous candidiasis (CMC).
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| Schneider, C., Docke, W.D., Zollner, T.M., Rose, L. (2008) Chronic Mouse Model of TMA-Induced Contact Hypersensitivity. J Invest Dermatol. Vol. 129(4):899-907.
Interleukin, IL4, IL12, IL13, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: IFN-gamma, IL-1beta, IL-4, IL-12, IL-13, TNF-alpha
Matrix tested: Mouse ear homogenates
Summary: In this study a chronic mouse model for atopic dermatitis was developed by comparing a standard acute trimellitic anhydride (TMA)-induced chronic hypersensitivity model in Balb/c mice with subacute and chronic models of TMA-induced ear inflammation.
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| Serra, A., Nuti, S., Tavarini, S., Sammicheli, C., Rosa, D., Saletti, G., Soldaini, E., Abrignani, S., Wack, A. (2008) Coligation of the Hepatitis C Virus Receptor CD81 with CD28 Primes Naive T Lymphocytes to Acquire Type 2 Effector Function. J Immunology. Vol. 181:174-185.
Interleukin, IL2, IL4, IL5, IL10, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor
Analytes tested using MSD assays: IFN-gamma, IL-2, IL-4, IL-5, IL-10, TNF-alpha
Matrix tested: Human peripheral blood mononuclear cell (PBMC) culture supernatants
Summary: In this study, a novel T-cell receptor independent activation pathway has been described for human T cells based on co-engagement of the costimulatory molecule CD28 and a number of tetraspanins including the HCV receptor CD81.
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| Sun, J., Schiffman, J., Raghunath, A., Ng Tang, D., Chen, H., Sharma, P. (2008) Concurrent decrease in IL-10 with development of immune-related adverse events in a patient treated with anti-CTLA-4 therapy. Cancer Immun. Vol. 8:9.
Interleukin, IL2, IL4, IL10, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g
Analytes tested using MSD assays: IFN-gamma, IL-2, IL-4, IL-10
Matrix tested: Human plasma
Summary: In this paper, immune related adverse events (irAEs) in patients treated with anti-CTLA-4 antibody have been investigated by measuring plasma cytokines in bladder cancer patients treated with anti-CTLA-4 therapy.
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| Thompson, W.L., Karpus W.J., Van Eldik, L.J. (2008) MCP-1-deficient mice show reduced neuroinflammatory responses and increased peripheral inflammatory responses to peripheral endotoxin insult. J Neuroinflam. Vol. 5:35.
Monocyte Chemotactic Protein
Analytes tested using MSD assays: MCP-1
Matrix tested: Mouse brain homogenates
Summary: This study examined the role of the chemokine MCP-1 in activation of the brain during acute systemic inflammation by testing the effects of inhibiting MCP-1 on brain inflammation using MCP-1 knock-out mice.
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| Toedter, G., Hayden, K., Wagner, C., Brodmerkel, C. (2008) Simultaneous Detection of Eight Analytes in Human Serum by Two Commercially Available Platforms for Multiplex Cytokine Analysis. Clin Vaccine Immunol. Vol. 15(1):42–48.
Interleukin, IL4, IL6, IL12p40, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, Monocyte Chemotactic Protein, MIP1ß, MIP1b, MIP1beta, vascular endothelial growth factor, macrophage inflammatory protein
Analytes tested using MSD assays: IFN-gamma, VEGF, IL-4, IL-6, IL-12p40, MIP-1beta, MCP-1, TNF-alpha
Matrix tested: Human serum
Summary: In this study, a comparison of two commercially available multiplex Platforms, MESO SCALE DISCOVERY and Pierce SearchLight technology was done in conjunction with standard ELISA to measure eight different cytokines in serum samples from subjects with ulcerative colitis, Crohn’s disease, rheumatoid arthritis (RA), and psoriasis. Spike recovery and the range of quantitation were also compared.
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| Tran, T.A., McCoy, M.K., Sporn, M.B., Tansey, M.G. (2008) The synthetic triterpenoid CDDO-methyl ester modulates microglial activities, inhibits TNF production, and provides dopaminergic neuroprotection. J Neuroinflammation. Vol. 5:14.
Interleukin, IL6, IL10, IL12, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, tumor necrosis factor
Analytes tested using MSD assays: IFN-gamma, IL-1beta, IL-6, IL-10, IL-12, KC / GRO / CXCL1, TNF-alpha
Matrix tested: Murine BV2 microglial cell culture supernatants
Summary: This study examined the cellular basis for the anti-inflammatory properties of a synthetic triterpenoid methyl-2-cyano-3,12-dioxooleana-1,9-dien-28-oate (CDDO-Me), its ability to modulate microglial activity and protect neurons against inflammation.
|
| Wolk, K.E., Lazarowski, E.R., Traylor, Z.P., Yu, E.N., Jewell, N.A., Durbin, R.K., Durbin, J.E., Davis, I.C. (2008) Influenza A virus inhibits alveolar fluid clearance in BALB/c mice. Am J Respir Crit Care Med. Vol. 178(9):969-76.
pro-inflammatory
Analytes tested using MSD assays: Proinflammatory cytokines and chemokines (not individually listed)
Matrix tested: Bronchoalveolar lavage fluid from BALB/c mice
Summary: Alveolar fluid clearance after Influenza A viral infection was tested in BALB/c mice.
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| Yang, B., Trump, R.P., Shen, Y., McNulty, J.A., Clifton, L.G., Stimpson, S.A., Lin, P., Pahel, G.L. (2008) RU486 did not exacerbate cytokine release in mice challenged with LPS nor in db/db mice. BMC Pharmacology. Vol. 8:7.
Interleukin, IL6, IL10, IL12, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, tumor necrosis factor
Analytes tested using MSD assays: IFN-gamma, IL-1beta, IL-6, IL-10, IL-12, KC / GRO / CXCL1, TNF-alpha
Matrix tested: Murine BV2 microglial cell culture supernatants
Summary: This study is based on the comparison of IL-13 expression in peripheral blood T-cells and eosinophils from asthma patients with healthy controls and attempts to identify an inflammatory stimulus for this expression.
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| Young, H.A. (2008) Cytokine Multiplex Analysis in Inflammation and Cancer. Methods and Protocols: ed. Kozlov, S.V. Volume 1: Experimental Models and Practical Approaches. Vol. 511: 85-105.
RNAse protection assay, high throughput genomics, PCR, MSD, Luminex, SearchLight, FASTQuant, Flow cytometry, FlowCytomix, ELISA
Summary: This article reviews the different commercial approaches that are available for the analysis of multiple cytokines in single samples at the protein and RNA levels.
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| Chen, J.H., Zhang, Z.M. Stebbins J.L., Zhang, X.Y., Hoffman, R., Moore, A., Pellecchia, M. (2007) A Fragment-Based Approach for the Discovery of Isoform-Specific p38 Inhibitors. ACS Chem Bio. Vol. 2(5):329-336.
TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor
Analytes tested using MSD assays: TNF-alpha
Matrix tested: Cell culture medium
Summary: In this paper, a new method for the discovery of novel kinase inhibitors has been developed based on the application of the interligand NOE (nuclear Overhauser effect) fragment-based approach. NMR spectroscopy was used for screening and structure determination.
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| Ghosh, T.K., Michelson, D.J., Solberg, J.C., Lipson, K.E., Inglefield, J.R., Alkan, S.S. (2007) TLR-TLR cross talk in human PBMC resulting in synergistic and antagonistic regulation of type-1 and 2 interferons, IL-12 and TNF-alpha. Int Immunopharmacol. Vol. 7(8):1111-21.
Interleukin, IL12p70, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, IFNalpha, IFN-a, IFNa, IFNa, IFN-a, IFNbeta, IFN-ß, IFNß, IFNb, IFN-b, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor
Analytes tested using MSD assays: IFN-alpha, IFN-beta, IFN-gamma, IL-12p70, TNF-alpha
Matrix tested: Human peripheral blood mononuclear cell (PBMC) culture supernatants
Summary: In this study, the effect of a combination of Toll-like receptor agonists on cytokine production was examined using human PBMCs.
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| Hagemann, T., Robinson, S.C., Thompson, R.G., Charles, K., Kulbe, H., Balkwill, F.R. (2007) Ovarian cancer cell-derived migration inhibitory factor enhances tumor growth, progression, and angiogenesis. Mol Cancer Ther. Vol. 6(7):1993-2002.
Interleukin, IL2, IL4, IL5, IL10, IL12, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, IL-10, IL-12, KC / GRO / CINC, TNF-alpha
Matrix tested: Ascites fluid from ovarian carcinoma patients, cell culture supernatants
Summary: This paper examined the mechanisms through which malignant cell-derived macrophage migration inhibitory factor (MIF) influences ovarian cancer growth and invasion.
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| Hall, J.P., Kurdi Y., Hsu S., Cuozzo J., Liu J., Telliez J.B., Seidl K.J., Winkler, A., Hu, Y., Green, N., Askew, G.R., Tam, S., Clark, J.D., Lin, L.L. (2007) Pharmacologic inhibition of tpl2 blocks inflammatory responses in primary human monocytes, synoviocytes, and blood. J Biol Chem. Vol. 282(46):33295-304.
Interleukin, IL8
Analytes tested using MSD assays: IL-8
Matrix tested: HeLa cell culture supernatants
Summary: In this study, several highly selective and potent small molecule inhibitors of Tpl2 (MAPKKK in the ERK and the Tpl2-MEK-ERK signaling pathways) activity to validate the functions of Tpl2 in human cells.
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| Holland, S.M., DeLeo, F.R., Elloumi, H.Z., Hsu, A.P., Uzel, G., Brodsky, N., Freeman, A.F., Demidowich, A., Davis, J., Turner, M.L., Anderson, V.L., Darnell, D.N., Welch, P.A., Kuhns, D.B., Frucht, D.M., Malech, H.L., Gallin, J.I., Kobayashi, S.D., Whitney, A.R., Voyich, J.M., Musser, J.M., Woellner, C., Schäffer, A.A., Puck, J.M., Grimbacher, B. (2007) STAT3 Mutations in the Hyper-IgE Syndrome. New England J Med. Vol. 357(16):1655-1658.
Interleukin, IL8
Analytes tested using MSD assays: Cytokines (type not specifically mentioned)
Matrix tested: Human peripheral blood mononuclear cell (PBMC) culture supernatants
Summary: This study investigated the phenotypes of patients suffering from autosomal dominant hyper-immunoglobulin E syndrome (HIES) to assay gene expression and in vitro cytokine production to identify relevant pathways.
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| Lutterloh, E.C., Opal, S.M., Pittman, D.D., Keith, J.C., Tan, X.Y., Clancy, B.M., Palmer, H., Milarski, K., Sun, Y., Palardy, J.E., Parejo, N.A., Kessimian, N. (2007) Inhibition of the RAGE products increases survival in experimental models of severe sepsis and systemic infection. Crit Care. Vol. 11(6):122.
Monocyte chemoattractant protein, Interleukin, IL6, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: IFN-gamma, IL-1beta, IL-6, MCP-1, TNF-alpha
Matrix tested: Mouse serum
Summary: The therapeutic role of RAGE inhibition was examined in the mouse cecal ligation and puncture (CLP) model of polymicrobial sepsis and a mouse model of systemic listeriosis.
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| Patou, J., Gevaert, P., Van Zele, T., Holtappels, G., van Cauwenberge, P., Bachert, C. (2007) Staphylococcus aureus enterotoxin B, protein A, and lipoteichoic acid stimulations in nasal polyps. J Allergy Clin Immunol. Vol. 121 (1):110-115.
Interleukin, IL2, IL4, IL5, IL8, IL10, IL12p70, IL13, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, IL-8, IL-10, IL-12p70, IL-13, TNF-a
Matrix tested: Human nasal tissue homogenates
Summary: This study investigated the modulatory effects of the S. aureus surface proteins SpA, LTA, and enterotoxin B (SEB) in nasal polyp tissue and tested the differences from normal nasal (control) tissue.
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| Ronkina, N., Kotlyarov, A., Dittrich-Breiholz, O., Kracht, M., Hitti, E., Milarski, K. (2007) The Mitogen-Activated Protein Kinase (MAPK)-Activated Protein Kinases MK2 and MK3 Cooperate in Stimulation of Tumor Necrosis Factor Biosynthesis and Stabilization of p38 MAPK. Mol Cell Biol. Vol. 27(1):180-181.
Monocyte chemoattractant protein, Interleukin, IL1, IL6, IL8, IL10, IL12p40, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, tumor necrosis factor, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, Granulocyte Macrophage Colony Stimulating Factor, regulated upon activation, normal T cell expressed and secreted
Analytes tested using MSD assays: IFN-gamma, IL-1, IL-6, IL-8, IL-10, IL-12p40, KC / GRO / CINC, RANTES, GM-CSF, MCP-1, TNF-alpha
Matrix tested: Mouse bone marrow-derived macrophage cell culture supernatants
Summary: MK2 and MK3 are protein kinases downstream of p38 mitogen-activated protein kinase (MAPK). In this paper, the physiological role of MK3 has been analyzed by gene targeting strategy in an MK2-free genetic background.
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| Salter, M., Biggadike, K., Matthews, J.L., West, M.R., Haase, M.V., Farrow, S.N., Uings, I.J, Gray, D.W. (2007) Pharmacological properties of the enhanced-affinity glucocorticoid fluticasone furoate in vitro and in an in vivo model of respiratory inflammatory disease. Am J Physiol Lung Cell Mol Physiol. Vol. 293(3):L660-7.
Tumor necrosis factor, TNFa, TNF-a, TNFalpha, TNFa, TNF-a
Analytes tested using MSD assays: TNF-alpha
Matrix tested: Human peripheral blood mononuclear cell (PBMC) culture supernatants
Summary: This study examined the properties of Fluticasone furoate (FF), a novel enhanced-affinity glucocorticoid for topical therapy for allergic rhinitis, with respect to its efficacy, selectivity, and tissue binding.
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| Seike, M., Yanaihara, N., Bowman, E.D., Zanetti, K.A., Budhu, A., Kumamoto, K., Mechanic, L.E., Matsumoto, S., Yokota, J., Shibata, T., Sugimura, H., Gemma, A., Kudoh, S., Wang, X.W., Harris, C.C. (2007) Use of a cytokine gene expression signature in lung adenocarcinoma and the surrounding tissue as a prognostic classifier. J Natl Cancer Inst. Vol. 15(99):1257-69.
Interleukin, IL2, IL8, IL10, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, tumor necrosis factor, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: IFN-gamma, IL-1beta, IL-2, IL-8, IL-10, TNF-alpha
Matrix tested: Human lung tissue homogenates
Summary: In this study, gene expression profiling of noncancerous lung tissue was done to test whether it could predict the metastatic capability of lung adenocarcinoma.
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| Sterling T.R., Martire, T., de Almeida, A.S., Ding, L., Greenberg, D.E., Moreira, L.A., Elloumi, H., Torres, A.P., Sant'Anna, C.C., Calazans, E., Paraguassu, G., Gebretsadik, T., Shintani, A., Miller, K., Kritski, A., Lapa e Silva, J.R., Holland, S.M. (2007) Immune function in young children with previous pulmonary or miliary/meningeal tuberculosis and impact of BCG vaccination. Pediatrics. Vol. 120(4):912-21.
Interleukin, IL8
Analytes tested using MSD assays: IL-8
Matrix tested: Human peripheral blood mononuclear cell (PBMC) culture supernatants
Summary: This paper investigated the immunologic factors associated with military / meningeal tuberculosis in young children.
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| Thomas, A., Laxton, C., Rodman, J., Myangar, N., Horscroft, N., Parkinson, T. (2007) Investigating Toll-like receptor agonists for potential to treat hepatitis C virus infection. Antimicrob Agents Chemother. Vol. 51(8):2969-78.
Interleukin, IL6, Interferon, IFNalpha, IFN-a2a, IFNa2a, IFNa2a, IFN-a2a, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, tumor necrosis factor
Analytes tested using MSD assays: IFN-alpha2a, IL-1beta, IL-6, TNF-alpha
Matrix tested: Human peripheral blood mononuclear cell (PBMC) culture supernatants
Summary: This paper examined the effects of agonists of Toll-like receptors 1 to 9 to see which ones are capable of inducing antiviral activity against HCV infection in human peripheral blood mononuclear cells (PBMCs).
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| Trifonova, R.T., Bajpai, M., Pasicznyk, J.M., Chandra, N., Doncel, G.F., Fichorova, R.N. (2007) Biomarkers of leukocyte traffic and activation in the vaginal mucosa. Biomarkers. Vol. 12(6):608-22.
vascular adhesion molecule, HIV-1
Analytes tested using MSD assays: E-selectin, VCAM-1
Matrix tested: Human cervicovaginal lavage (CVL) fluid
Summary: This paper is based on the identification of non-invasive soluble biomarkers of vascular and leukocyte activation for the evaluation of compound-induced cervicovaginal mucosal inflammation.
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| Athauda, G., Giubellino, A., Coleman, J.A., Horak, C., Steeg, P.S., Lee, M.J., Trepel, J., Wimberly, J., Sun, J., Coxon, A., Burgess, T.L., Bottaro, D.P. (2006) c-Met Ectodomain Shedding Rate Correlates with Malignant Potential. Clin Cancer Res. Vol. 12(14):4154-4162.
Hepatocyte growth factor receptor, cMet, cancer
Analytes tested using MSD assays: Customer developed assay on MSD Streptavidin plates for ectodomain of c-Met
Matrix tested: Cell culture supernatants
Summary: This study tested the hypothesis that c-Met overexpression in cancer might result in increased ectodomain shedding, which in turn could be a biomarker of tumor progression.
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| Bower, W.A., Culver, D.H., Castor, D., Wu, Y., James, V.N., Zheng, H., Hammer, S., Kuhnert, W.L., Williams, I.T., Bell, B.P., Vlahov, D., Dezzutti, C.S. (2006) Changes in hepatitis C virus (HCV) viral load and interferon-alpha levels in HIV/HCV-coinfected patients treated with highly active antiretroviral therapy. J Acquir Immune Defic Syndr. Vol. 42(3):293-7.
Interferon, IFNalpha, IFN-a, IFNa, IFNa, IFN-a
Analytes tested using MSD assays: IFN-alpha
Matrix tested: Human serum
Summary: In this study, the authors evaluated the impact of Highly Active Antiretroviral Therapy (HAART) on HCV RNA titer and transaminase levels in HIV / HCV-coinfected patients.
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| Fichorova, R.N., Trifonova, R.T., Gilbert, R.O., Costello, C.E., Hayes, G.R., Lucas, J.J., Singh, B.N. (2006) Trichomonas vaginalis Lipophosphoglycan Triggers a Selective Upregulation of Cytokines by Human Female Reproductive Tract Epithelial Cells. Infection and Immunity. Vol. 74(10):5773-5779.
Interleukin, IL6, IL8, IL10, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, MIP3alpha, MIP3a, MIP-3a, MIP-3a, MIP3a, macrophage inflammatory protein 3 alpha
Analytes tested using MSD assays: IL-1beta, IL-6, IL-8, IL-10, MIP-3alpha, TNF-alpha
Matrix tested: Cell culture supernatants
Summary: This study tested whether lipophosphoglycan (LPG), the main cell surface glycoconjugate of T. vaginalis, initiates cytokine secretion by host cells.
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| Ge, Y., Montano, I., Rustici, G., Freebern, W.J., Haggerty, C.M., Cui, W., Ponciano-Jackson, D., Chandramouli, G.V., Gardner, E.R., Figg, W.D., Abu-Asab, M., Tsokos, M., Jackson, S.H., Gardner. K. (2006) Selective leukemic-cell killing by a novel functional class of thalidomide analogs. Blood. Vol. 108:4126-35.
Granulocyte macrophage colony stimulating factor, Interleukin, IL2, IL4, IL5, IL6, IL8, IL10, IL12p70, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, tumor necrosis factor, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß
Analytes tested using MSD assays: GM-CSF, IL1beta, IL2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, TNF-alpha
Matrix tested: Cell culture supernatants
Summary: This study is based on the profiling of transcriptional and signal transduction pathways of thalidomide derivatives to identify a novel functional class of thalidomide analogs that act through direct manipulation of the cellular redox state to selectively kill leukemic cells.
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| Ghosh, T.K., Michelson, D.J., Fink, J., Solberg, J.C., Inglefield, J.R., Hook, D., Gupta, S.K., Gibson, S., Alkan, S.S. (2006) Toll-like receptor (TLR) 2-9 agonists-induced cytokines and chemokines: I. Comparison with T cell receptor-induced responses. Cell Immunol. Vol. 243(1):48-57.
Interferon, IFNalpha, IFN-a, IFNa, IFNa, IFN-a, IFNbeta, IFN-ß, IFNß, IFNb, IFN-b, Interleukin, IL2, IL4, IL8, IL10, IL12p40, IL12p70, IL13, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, IL8, Monocyte Chemotactic Protein, macrophage inflammatory protein 1 beta, MIP1ß, MIP1b, MIP1beta
Analytes tested using MSD assays: IFN-alpha, IFN-beta, IFN-gamma, IL-1beta, IL-2, IL-4, IL-8, IL-10, IL-12p40, IL-12p70, IL-13, TNF-alpha, MCP-1, MIP-1beta, RANTES
Matrix tested: Human peripheral blood mononuclear cell (PBMC) culture supernatants
Summary: In this study, the cytokine and chemokine profiles generated by individual TLR agonists, TLR–TLR and TLR-T cell receptor (TCR) interactions were investigated.
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| Pulichino, A.M., Rowland, S., Wu, T., Clark, P., Xu, D., Mathieu, M.C., Riendeau, D., Audoly, L.P. (2006) Prostacyclin antagonism reduces pain and inflammation in rodent models of hyperalgesia and chronic arthritis. J Pharmacol Exp Ther. Vol. 319(3):1043-50.
Tumor necrosis factor, TNFa, TNF-a, TNFalpha, TNFa, TNF-a
Analytes tested using MSD assays: TNF-alpha
Matrix tested: Rat whole blood
Summary: In this paper, the role of Prostaglandin type, PGI2, has been examined in a rat model of osteoarthritis (OA) and rheumatoid arthritis using an antagonist to PGI2 receptor an mice that are deficient of this receptor.
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| Ryan, S., Taylor, C.T., McNicholas, W.T. (2006) Predictors of elevated nuclear factor-kappaB-dependent genes in obstructive sleep apnea syndrome. Am J Res and Crit Care Med. Vol. 174(7):824-30.
Interleukin, IL1, IL6, IL8, IL10, IL12, Interferon, IFNgamma, IFN-g, IFNg, IFNg, IFN-g, tumor necrosis factor, TNFa, TNF-a, TNFalpha, TNFa, TNF-a
Analytes tested using MSD assays: IFN-gamma, IL-1, IL-6, IL-8, IL-10, IL-12, TNF-alpha
Matrix tested: Human serum
Summary: The focus of this study was to determine, with a particular emphasis on TNF-alpha, the clinical predictors of elevated proinflammatory cytokines in obstructive sleep apnea syndrome (OSAS).
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| Trifonova, R.T., Pasicznyk, J.M., Fichorova, R.N. (2006) Biocompatibility of solid-dosage forms of anti-human immunodeficiency virus type 1 microbicides with the human cervicovaginal mucosa modeled ex vivo. Antimicrobial Agents and Chemotherapy. Vol. 50(12):4005-10.
Interleukin, IL6, IL8, tumor necrosis factor, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, Interferon Inducible Protein 10
Analytes tested using MSD assays: IL-1beta, IL-6, IL-8, IP-10, TNF-alpha
Matrix tested: Human serum
Summary: In this study, a topical anti-HIV-1 microbicide, cellulose acetate 1,2-benzenedicarboxylate (CAP) and other over-the-counter (OTC) vaginal products were evaluated for their impact on inflammatory mediators signifying potential HIV-1-enhancing risk factors.
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| Freebern, W.J., Haggerty, C.M., Montano, I., McNutt, M.C., Collins, I., Graham, A., Chandramouli, G.V.R., Stewart, D.H., Biebuyck, H.A., Taub, D.D., Gardner, K. (2005) Pharmacologic profiling of transcriptional targets deciphers promoter logic. Pharmacogenomics J. Vol. 5(5):305-323.
Granulocyte macrophage colony stimulating factor, Interleukin, IL2, IL4, IL5, IL6, IL8, IL10, IL12p70, IL-1b, IL1beta, IL1b, IL-1ß, IL1ß, tumor necrosis factor, TNFa, TNF-a, TNFalpha, TNFa, TNF-a
Analytes tested using MSD assays: GM-CSF, IL-1beta, IL2, IL4, IL5, IL6, IL8, IL10, IL12p70, TNF-alpha
Matrix tested: Cell culture supernatants
Summary: In this study, a novel transcriptional approach for profiling the activation of multiple transcriptional targets against arrays of therapeutic and signal transducing agents has been described.
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| Han, S., Zhou, V., Pan, S., Liu, Y., Hornsby, M., McMullan, D., Klock, H.E., Haugen, J., Lesley, S.A., Gray, N., Caldwell, J., Gu, X.J. (2005) Identification of coumarin derivatives as a novel class of allosteric MEK1 inhibitors. Bioorg Med Chem Lett. Vol. 15(24):5467-73.
Tumor necrosis factor, TNFa, TNF-a, TNFalpha, TNFa, TNF-a, ERK, MAPK
Analytes tested using MSD assays: TNF-alpha
Matrix tested: Cell culture supernatants
Summary: This paper is based on the identification of novel inhibitors of the ERK/MAPK pathway using a TR-FRET-based in vitro coupling assay.
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* Pub Med (www.pubmed.gov) is a service of the U.S. National Library of Medicine and the National Institutes of Health
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